Detachment and flow cytometric quantification of seagrass-associated bacteria

Trevathan-Tackett,S, Macreadie,P, Ralph,P and Seymour,J 2014, Detachment and flow cytometric quantification of seagrass-associated bacteria, Journal of Microbiological Methods, vol. 102, pp. 23-25, doi: 10.1016/j.mimet.2014.04.008.

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Title Detachment and flow cytometric quantification of seagrass-associated bacteria
Author(s) Trevathan-Tackett,S
Macreadie,PORCID iD for Macreadie,P orcid.org/0000-0001-7362-0882
Ralph,P
Seymour,J
Journal name Journal of Microbiological Methods
Volume number 102
Start page 23
End page 25
Total pages 3
Publisher Elsevier
Place of publication Amsterdam , Netherlands
Publication date 2014-07
ISSN 1872-8359
Keyword(s) Bacterial detachment
Enumeration
Flow cytometry
Microbe
Seagrass
Seagrass-associated bacteria
Summary A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0×10(5) and 8.0×10(6)cells mg(-1) DW tissue.
Language eng
DOI 10.1016/j.mimet.2014.04.008
Field of Research 060205 Marine and Estuarine Ecology (incl Marine Ichthyology)
Socio Economic Objective 960903 Coastal and Estuarine Water Management
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30070709

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