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Studies on the interaction between malanin and metal ions by fluorescence spectra methods

Yuan, Y., Zhang, W., Dai, J-H., Liu, X-F., Wang, H-B., Barrow, Colin J. and Yang, Wenrong 2014, Studies on the interaction between malanin and metal ions by fluorescence spectra methods, Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis, vol. 34, no. 12, pp. 3297-3300, doi: 10.3964/j.issn.1000-0593(2014)12-3297-04.

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Title Studies on the interaction between malanin and metal ions by fluorescence spectra methods
Author(s) Yuan, Y.
Zhang, W.
Dai, J-H.
Liu, X-F.
Wang, H-B.
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Journal name Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis
Volume number 34
Issue number 12
Start page 3297
End page 3300
Total pages 3
Publisher Peking University Press
Place of publication Beijing, China
Publication date 2014-12
ISSN 1000-0593
Keyword(s) Fluorescence quenching
Fluorescence spectrum
Malanin
Metal ion
Science & Technology
Technology
Spectroscopy
OXYGEN
VENOM
Summary A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces. Effects of copper ion, silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied. The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence. The dissociation constant of them from malanin and apo-malanin were about 2.37×10-4 and 2.66×10-4 mol·L-1, respectively. The silver ion did not have quenching action on malanin fluorescence, but it had statistic quenching effect on apo-malanin fluorescence, and its dissociation constant was 2.37×10-4 mol·L-1. Calcium ion did not have quenching action on malanin and apo-malanin fluorescence. It plays an important role in keeping malanin natural conformation.
Language chi
DOI 10.3964/j.issn.1000-0593(2014)12-3297-04
Field of Research 030101 Analytical Spectrometry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Peking University Press
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30070978

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.