Optimization of zeaxanthin and β-carotene extraction from Chlorella saccharophila isolated from New Zealand marine waters

Singh, Dilip, Barrow, Colin J., Mathur, Anshu S., Tuli, Deepak K. and Puri, Munish 2015, Optimization of zeaxanthin and β-carotene extraction from Chlorella saccharophila isolated from New Zealand marine waters, Biocatalysis and agricultural biotechnology, vol. 4, no. 2, pp. 166-173, doi: 10.1016/j.bcab.2015.02.001.

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Title Optimization of zeaxanthin and β-carotene extraction from Chlorella saccharophila isolated from New Zealand marine waters
Author(s) Singh, Dilip
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Mathur, Anshu S.
Tuli, Deepak K.
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Biocatalysis and agricultural biotechnology
Volume number 4
Issue number 2
Start page 166
End page 173
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-04
ISSN 1878-8181
Keyword(s) Central composite design
Marine algae
Response surface methodology
Ultrasonication
Summary Zeaxanthin is an important natural pigment which has found commercial application in food and nutritional supplements. Its potential widespread use requires an easy and effective extraction methodology for obtaining higher yields. Extraction from Chlorella sp. under optimized conditions demonstrated a marked reduction in extraction time (13.48min) compared with a control experiment (30min). The extraction conditions such as solvent/cell dry weight (CDW) ratio, power, pulse, time and their combinations were optimized using response surface methodology (RSM). Almost all the variables were shown significantly (p-value <0.05) affect the carotenoid yield. Significant interaction (p-value <0.05) was observed with a substantial effect on zeaxanthin yield for solvent/CDW ratio and power, as well as power and time, whereas the β-carotene control exhibited significant interaction between solvent/CDW ratio and pulse, as well as between pulse and time. The R 2-value approached unity in both models, demonstrating their accuracy. Data obtained from these interactions were used to construct 3D response plots. Solvent/CDW ratio of 67.38μlmg-1, power 27.82% (total power 500W), pulse length of 19.7s and time 13.48min were found to be the optimized conditions for zeaxanthin (11.2mgg-1) and β-carotene (4.98mgg-1) extraction.
Language eng
DOI 10.1016/j.bcab.2015.02.001
Field of Research 100303 Fermentation
Socio Economic Objective 860105 Nutraceuticals and Functional Foods
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30070987

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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