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Lipidomic analysis of Arabidopsis seed genetically engineered to contain DHA

Zhou,X-R, Callahan,DL, Shrestha,P, Liu,Q, Petrie,JR and Singh,SP 2014, Lipidomic analysis of Arabidopsis seed genetically engineered to contain DHA, Frontiers in plant science, vol. 5, pp. 1-12, doi: 10.3389/fpls.2014.00419.

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Title Lipidomic analysis of Arabidopsis seed genetically engineered to contain DHA
Author(s) Zhou,X-R
Callahan,DLORCID iD for Callahan,DL
Journal name Frontiers in plant science
Volume number 5
Start page 1
End page 12
Publisher Frontiers Research Foundation
Place of publication Lausanne, Switzerland
Publication date 2014-09-01
ISSN 1664-462X
Keyword(s) Arabidopsis
Metabolic engineering
Summary Metabolic engineering of omega-3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFA) in oilseeds has been one of the key targets in recent years. By expressing a transgenic pathway for enhancing the synthesis of the ω3 LC-PUFA docosahexaenoic acid (DHA) from endogenous α-linolenic acid (ALA), we obtained the production of fish oil-like proportions of DHA in Arabidopsis seed oil. Liquid chromatography-mass spectrometry (LC-MS) was used to characterize the triacylglycerol (TAG), diacylglycerol (DAG) and phospholipid (PL) lipid classes in the transgenic and wild type Arabidopsis seeds at both developing and mature stages. The analysis identified the appearance of several abundant DHA-containing phosphatidylcholine (PC), DAG and TAG molecular species in mature seeds. The relative abundances of PL, DAG, and TAG species showed a preferred combination of LC-PUFA with ALA in the transgenic seeds, where LC-PUFA were esterified in positions usually occupied by 20:1ω9. Trace amounts of di-DHA PC and tri-DHA TAG were identified and confirmed by high resolution MS/MS. Studying the lipidome in transgenic seeds provided insights into where DHA accumulated and combined with other fatty acids of neutral and phospholipids from the developing and mature seeds. © 2014 Zhou, Callahan, Shrestha, Liu, Petrie and Singh.
Language eng
DOI 10.3389/fpls.2014.00419
Field of Research 060702 Plant Cell and Molecular Biology
030101 Analytical Spectrometry
Socio Economic Objective 820499 Summer Grains and Oilseeds not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Frontiers Research Foundation
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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