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Foxp3 expression in macrophages associated with RENCA tumors in mice.

Devaud, Christel, Yong, Carmen SM, John, Liza B, Westwood, Jennifer A, Duong, Connie PM, House, Colin M, Denoyer, Delphine, Li, Jason, Darcy, Phillip K and Kershaw, Michael H 2014, Foxp3 expression in macrophages associated with RENCA tumors in mice., PLoS One, vol. 9, no. 9, pp. 1-8, doi: 10.1371/journal.pone.0108670.

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Title Foxp3 expression in macrophages associated with RENCA tumors in mice.
Author(s) Devaud, Christel
Yong, Carmen SM
John, Liza B
Westwood, Jennifer A
Duong, Connie PM
House, Colin M
Denoyer, Delphine
Li, Jason
Darcy, Phillip K
Kershaw, Michael H
Journal name PLoS One
Volume number 9
Issue number 9
Article ID e108670
Start page 1
End page 8
Total pages 8
Publisher PLoS
Place of publication San Francisco, Calif.
Publication date 2014
ISSN 1932-6203
Summary The transcription factor Foxp3 represents the most specific functional marker of CD4+ regulatory T cells (TRegs). However, previous reports have described Foxp3 expression in other cell types including some subsets of macrophages, although there are conflicting reports and Foxp3 expression in cells other than Treg is not well characterized. We performed detailed investigations into Foxp3 expression in macrophages in the normal tissue and tumor settings. We detected Foxp3 protein in macrophages infiltrating mouse renal cancer tumors injected subcutaneously or in the kidney. Expression was demonstrated using flow cytometry and Western blot with two individual monoclonal antibodies. Further analyses confirmed Foxp3 expression in macrophages by RT PCR, and studies using ribonucleic acid-sequencing (RNAseq) demonstrated a previously unknown Foxp3 messenger (m)RNA transcript in tumor-associated macrophages. In addition, depletion of Foxp3+ cells using diphtheria toxin in Foxp3DTR mice reduced the frequency of type-2 macrophages (M2) in kidney tumors. Collectively, these results indicate that tumor-associated macrophages could express Foxp3.
Language eng
DOI 10.1371/journal.pone.0108670
Field of Research 111201 Cancer Cell Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30071492

Document type: Journal Article
Collections: School of Life and Environmental Sciences
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.