Off-line two-dimensional liquid chromatography for metabolomics: an example using Agaricus bisporus mushrooms exposed to UV irradiation

Pandohee,J, Stevenson,PG, Conlan, Xavier A, Zhou,X-R and Jones,OAH 2015, Off-line two-dimensional liquid chromatography for metabolomics: an example using Agaricus bisporus mushrooms exposed to UV irradiation, Metabolomics, vol. 11, no. 4, pp. 939-951, doi: 10.1007/s11306-014-0749-4.

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Title Off-line two-dimensional liquid chromatography for metabolomics: an example using Agaricus bisporus mushrooms exposed to UV irradiation
Author(s) Pandohee,J
Stevenson,PG
Conlan, Xavier AORCID iD for Conlan, Xavier A orcid.org/0000-0003-0829-0551
Zhou,X-R
Jones,OAH
Journal name Metabolomics
Volume number 11
Issue number 4
Start page 939
End page 951
Total pages 13
Publisher Springer
Place of publication Berlin, Germany
Publication date 2015-08
ISSN 1573-3882
1573-3890
Keyword(s) HPLC
Metabonomics
Natural products
Reversed phase
Separation science
Vitamin D
Summary It has previously been shown that irradiation with UV light increases the vitamin D content of certain mushroom species, but the effect on other nutrients is unknown, and is difficult to assess due to the complexity of the sample matrix. Here, an offline reversed phase × reversed phase two-dimensional liquid chromatography methodology was developed and applied to Agaricus bisporus mushrooms in order to demonstrate the potential of the technique and assess the effect of UV irradiation on the mushroom’s metabolic profile. The method allowed the detection of 158 peaks in a single analytical run. A total of 51 compounds including sugars, amino acids, organic and fatty acids and phenolic compounds were identified using certified reference standards. After irradiation of the mushrooms with UV for 30 s the number of peaks detected decreased from 158 to 150; 47 compounds increased in concentration while 72 substances decreased. This is the first time that two-dimensional liquid chromatography has been carried out for the metabolomic analysis of mushrooms. The data provide an overview of the gain/loss of nutritional value of the mushrooms following UV irradiation and demonstrate that the increased peak capacity and separation space of two-dimensional liquid chromatography has great potential in metabolomics.
Language eng
DOI 10.1007/s11306-014-0749-4
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
Copyright notice ©2014, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30071582

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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