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Optical coherence tomography : age estimation of Calliphora vicina pupae in vivo?

Brown,K and Harvey,M 2014, Optical coherence tomography : age estimation of Calliphora vicina pupae in vivo?, Forensic science international, vol. 242, pp. 157-161, doi: 10.1016/j.forsciint.2014.07.001.

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Title Optical coherence tomography : age estimation of Calliphora vicina pupae in vivo?
Author(s) Brown,K
Harvey,MORCID iD for Harvey,M orcid.org/0000-0002-4047-7845
Journal name Forensic science international
Volume number 242
Start page 157
End page 161
Total pages 5
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2014-09
ISSN 1872-6283
Keyword(s) Age estimation
Calliphora vicina
Forensic entomology
Microscopy
Optical coherence tomography
Pupae
Science & Technology
Life Sciences & Biomedicine
Medicine, Legal
Legal Medicine
MAGNETIC-RESONANCE MICROSCOPY
GENE-EXPRESSION
DIPTERA CALLIPHORIDAE
POSTMORTEM INTERVAL
COMPUTED TOMOGRAPHY
MORPHOLOGY
METAMORPHOSIS
BRAIN
HEART
Summary Necrophagous blowfly pupae are valuable contributors to the estimation of post-mortem interval, should an accurate age estimate be obtained. At present, this is reliant on a combination of rearing and destructive methods conducted on preserved samples, including morphological observation and gene expression analyses. This study demonstrates the use of optical coherence tomography (OCT) as a tool for in vivo morphological observation and pupal age estimation. Using a Michelson OCT microscope, alive and preserved four and ten-day old Calliphora vicina pupae were scanned in different orientations. Two and three-dimensional images were created. Morphological characteristics such as the brain, mouthparts and legs were identifiable in both living and preserved samples, with distinct differences noted between the two ages. Absorption of light by the puparium results in a vertical resolution of 1-2 mm, preventing observation of deeper tissues. The use of contrast agents or a longer wavelength laser would improve the images obtainable. At present, the data suggests OCT provides a primary view of external and internal morphology, which can be used to distinguish younger and older pupae for further analysis of age and PMI estimation.
Language eng
DOI 10.1016/j.forsciint.2014.07.001
Field of Research 069901 Forensic Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30071720

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