You are not logged in.
Openly accessible

A simple microfluidic chip design for fundamental bioseparation

Chan,AS, Danquah,MK, Agyei,D, Hartley,PG and Zhu,Y 2014, A simple microfluidic chip design for fundamental bioseparation, Journal of analytical methods in chemistry, vol. 2014, pp. 1-6, doi: 10.1155/2014/175457.

Attached Files
Name Description MIMEType Size Downloads
agyei-simplemicrofluidic-2014.pdf Published version application/pdf 1.22MB 52

Title A simple microfluidic chip design for fundamental bioseparation
Author(s) Chan,AS
Danquah,MK
Agyei,DORCID iD for Agyei,D orcid.org/0000-0003-2280-4096
Hartley,PG
Zhu,Y
Journal name Journal of analytical methods in chemistry
Volume number 2014
Start page 1
End page 6
Publisher Hindawi Publishing Corporation
Place of publication New York, NY
Publication date 2014
ISSN 2090-8865
Summary A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.
Language eng
DOI 10.1155/2014/175457
Field of Research 090404 Membrane and Separation Technologies
100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 869899 Environmentally Sustainable Manufacturing not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Hindawi Publishing Corporation
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30071774

Document type: Journal Article
Collections: School of Life and Environmental Sciences
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 3 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 65 Abstract Views, 51 File Downloads  -  Detailed Statistics
Created: Tue, 07 Apr 2015, 09:01:33 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.