A parametric study of a monolithic microfluidic system for on-chip biomolecular separation

Chan, Alan S., Danquah, Michael K., Agyei, Dominic, Hartley, Patrick G. and Zhu, Yonggang 2014, A parametric study of a monolithic microfluidic system for on-chip biomolecular separation, Separation science and technology, vol. 49, no. 6, pp. 854-860, doi: 10.1080/01496395.2013.872144.

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Title A parametric study of a monolithic microfluidic system for on-chip biomolecular separation
Author(s) Chan, Alan S.
Danquah, Michael K.
Agyei, DominicORCID iD for Agyei, Dominic orcid.org/0000-0003-2280-4096
Hartley, Patrick G.
Zhu, Yonggang
Journal name Separation science and technology
Volume number 49
Issue number 6
Start page 854
End page 860
Total pages 8
Publisher Taylor and Francis
Place of publication Abigndon, Eng.
Publication date 2014
ISSN 0149-6395
Keyword(s) biomolecules
stationary phase
Summary A microfabricated poly(dimethylsiloxane) (PDMS) chip containing channel filled with polymer monolith has been developed for on-chip biomolecule separation. Methacrylate monolithic polymers were prepared by photo-initiated polymerization within the channel to serve as a continuous stationary phase. The monolithic polymer was functionalized with a weak anion-exchange ligand, and key parameters affecting the binding characteristics of the system were investigated. The total binding capacity was unaffected by the flow rate of the mobile phase but varied significantly with changes in ionic strength and pH of the binding buffer. The binding capacity decreased with increasing buffer ionic strength, and this is due to the limited available binding sites for protein adsorption resulting from cationic shielding effect. Similarly, the binding capacity decreased with decreasing buffer pH towards the isoelectric point of the protein. A protein mixture, BSA and ovalbumin, was used to illustrate the capacity of the methacrylate-based microfluidic chip for rapid biomolecule separation.
Language eng
DOI 10.1080/01496395.2013.872144
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
090404 Membrane and Separation Technologies
Socio Economic Objective 869899 En
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2014, Taylor and Francis
Persistent URL http://hdl.handle.net/10536/DRO/DU:30071775

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