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CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria

Lau,LS, Fernandez-Ruiz,D, Mollard,V, Sturm,A, Neller,MA, Cozijnsen,A, Gregory,JL, Davey,GM, Jones,CM, Lin,YH, Haque,A, Engwerda,CR, Nie,CQ, Hansen,DS, Murphy,KM, Papenfuss,AT, Miles,JJ, Burrows,SR, de Koning-Ward,T, McFadden,GI, Carbone,FR, Crabb,BS and Heath,WR 2014, CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria, PLoS pathogens, vol. 10, no. 5, pp. 1-16, doi: 10.1371/journal.ppat.1004135.

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Title CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria
Author(s) Lau,LS
Fernandez-Ruiz,D
Mollard,V
Sturm,A
Neller,MA
Cozijnsen,A
Gregory,JL
Davey,GM
Jones,CM
Lin,YH
Haque,A
Engwerda,CR
Nie,CQ
Hansen,DS
Murphy,KM
Papenfuss,AT
Miles,JJ
Burrows,SR
de Koning-Ward,TORCID iD for de Koning-Ward,T orcid.org/0000-0001-5810-8063
McFadden,GI
Carbone,FR
Crabb,BS
Heath,WR
Journal name PLoS pathogens
Volume number 10
Issue number 5
Start page 1
End page 16
Publisher Public Library of Science
Place of publication San Francisco, CA
Publication date 2014-05
ISSN 1553-7374
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Microbiology
Parasitology
Virology
EXPERIMENTAL CEREBRAL MALARIA
CD8-ALPHA(+) DENDRITIC CELLS
HERPES-SIMPLEX VIRUS
DRAINING LYMPH-NODES
PLASMODIUM-BERGHEI
PROTECTIVE IMMUNITY
CUTTING EDGE
PATHOGENESIS
IMMUNIZATION
MICE
Summary To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. T cells from this line, termed PbT-I T cells, were able to respond to blood-stage infection by PbA and two other rodent malaria species, P. yoelii XNL and P. chabaudi AS. These PbT-I T cells were also able to respond to sporozoites and to protect mice from liver-stage infection. Examination of the requirements for priming after intravenous administration of irradiated sporozoites, an effective vaccination approach, showed that the spleen rather than the liver was the main site of priming and that responses depended on CD8α+ dendritic cells. Importantly, sequential exposure to irradiated sporozoites followed two days later by blood-stage infection led to augmented PbT-I T cell expansion. These findings indicate that PbT-I T cells are a highly versatile tool for studying multiple stages and species of rodent malaria and suggest that cross-stage reactive CD8+ T cells may be utilized in liver-stage vaccine design to enable boosting by blood-stage infections.
Language eng
DOI 10.1371/journal.ppat.1004135
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, PLoS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30072022

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.