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Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise

Mason,RR, Meex,RC, Russell,AP, Canny,BJ and Watt,MJ 2014, Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise, PLoS one, vol. 9, no. 7, pp. 1-9, doi: 10.1371/journal.pone.0103062.

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Title Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise
Author(s) Mason,RR
Meex,RC
Russell,APORCID iD for Russell,AP orcid.org/0000-0002-7323-9501
Canny,BJ
Watt,MJ
Journal name PLoS one
Volume number 9
Issue number 7
Start page 1
End page 9
Publisher Public Library of Science
Place of publication San Francisco, CA
Publication date 2014-07
ISSN 1932-6203
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ADIPOSE TRIGLYCERIDE LIPASE
HORMONE-SENSITIVE LIPASE
MODERATE-INTENSITY EXERCISE
CHANARIN-DORFMAN-SYNDROME
PERILIPIN 5
LIPID DROPLETS
FATTY-ACID
TRIACYLGLYCEROL LIPASE
MEDIATED LIPOLYSIS
INSULIN-RESISTANCE
Summary Lipolysis involves the sequential breakdown of fatty acids from triacylglycerol and is increased during energy stress such as exercise. Adipose triglyceride lipase (ATGL) is a key regulator of skeletal muscle lipolysis and perilipin (PLIN) 5 is postulated to be an important regulator of ATGL action of muscle lipolysis. Hence, we hypothesized that non-genomic regulation such as cellular localization and the interaction of these key proteins modulate muscle lipolysis during exercise. PLIN5, ATGL and CGI-58 were highly (>60%) colocated with Oil Red O (ORO) stained lipid droplets. PLIN5 was significantly colocated with ATGL, mitochondria and CGI-58, indicating a close association between the key lipolytic effectors in resting skeletal muscle. The colocation of the lipolytic proteins, their independent association with ORO and the PLIN5/ORO colocation were not altered after 60 min of moderate intensity exercise. Further experiments in cultured human myocytes showed that PLIN5 colocation with ORO or mitochondria is unaffected by pharmacological activation of lipolytic pathways. Together, these data suggest that the major lipolytic proteins are highly expressed at the lipid droplet and colocate in resting skeletal muscle, that their localization and interactions appear to remain unchanged during prolonged exercise, and, accordingly, that other post-translational mechanisms are likely regulators of skeletal muscle lipolysis.
Language eng
DOI 10.1371/journal.pone.0103062
Field of Research 110699 Human Movement and Sports Science not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, PLoS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30072023

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.