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Phosphate absorption across multiple epithelia in the Pacific hagfish (Eptatretus stoutii)

Schultz,AG, Guffey,SC, Clifford,AM and Goss,GG 2014, Phosphate absorption across multiple epithelia in the Pacific hagfish (Eptatretus stoutii), American journal of physiology : regulatory, integrative and comparative physiology, vol. 307, no. 6, pp. R643-R652, doi: 10.1152/ajpregu.00443.2013.

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Title Phosphate absorption across multiple epithelia in the Pacific hagfish (Eptatretus stoutii)
Author(s) Schultz,AGORCID iD for Schultz,AG orcid.org/0000-0002-7333-7646
Guffey,SC
Clifford,AM
Goss,GG
Journal name American journal of physiology : regulatory, integrative and comparative physiology
Volume number 307
Issue number 6
Start page R643
End page R652
Total pages 10
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2014-09-15
ISSN 0363-6119
1522-1490
Summary Inorganic phosphate (Pi) is an essential nutrient for all organisms, but in seawater, Pi is a limiting nutrient. This study investigated the primary mechanisms of Pi uptake in Pacific hagfish (Eptatretus stoutii) using ex vivo physiological and molecular techniques. Hagfish were observed to have the capacity to absorb Pi from the environment into at least three epithelial surfaces: the intestine, skin, and gill. Pi uptake in all tissues was concentration dependent, and saturable Pi transport was observed in the skin and gill at <2.0 mmol/l Pi. Gill and intestinal Pi uptake was sodium dependent, but Pi uptake into the skin increased under low sodium conditions. Gill Pi transport exhibited an apparent affinity constant ∼0.23–0.6 mmol/l Pi. A complete sequence of a type II sodium phosphate cotransporter (Slc34a) was obtained from the hagfish gill. Phylogenetic analysis of the hagfish Slc34a transporter indicates that it is earlier diverging than, and/or ancestral to, the other identified vertebrate Slc34a transporters (Slc34a1, Slc34a2, and Slc34a3). With the use of RT-PCR, the hagfish Slc34a transcript was detected in the intestine, skin, gill, and kidney, suggesting that this may be the transporter involved in Pi uptake into multiple epithelia in the hagfish. This is the first measurement of Pi uptake across the gill or skin of any vertebrate animal and first sodium phosphate cotransporter identified in hagfish.
Language eng
DOI 10.1152/ajpregu.00443.2013
Field of Research 060604 Comparative Physiology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30072084

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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