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Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition

Nadiminti, Pavani P., Rookes, James E., Boyd, Ben J. and Cahill, David M. 2015, Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition, Protoplasma, vol. 252, no. 6, pp. 1475-1486, doi: 10.1007/s00709-015-0777-6.

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Title Confocal laser scanning microscopy elucidation of the micromorphology of the leaf cuticle and analysis of its chemical composition
Author(s) Nadiminti, Pavani P.
Rookes, James E.ORCID iD for Rookes, James E. orcid.org/0000-0003-1479-2339
Boyd, Ben J.
Cahill, David M.ORCID iD for Cahill, David M. orcid.org/0000-0002-2556-0528
Journal name Protoplasma
Volume number 252
Issue number 6
Start page 1475
End page 1486
Total pages 12
Publisher Springer
Place of publication Berlin, Germany
Publication date 2015-11
ISSN 1615-6102
Keyword(s) Confocal laser scanning microscopy (CLSM)
Cuticle
Epicuticular waxes
Image analysis
Micromorphology
Optical microscopy
Summary Electron microscopy techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) have been invaluable tools for the study of the micromorphology of plant cuticles. However, for electron microscopy, the preparation techniques required may invariably introduce artefacts in cuticle preservation. Further, there are a limited number of methods available for quantifying the image data obtained through electron microscopy. Therefore, in this study, optical microscopy techniques were coupled with staining procedures and, along with SEM were used to qualitatively and quantitatively assess the ultrastructure of plant leaf cuticles. Leaf cryosections of Triticum aestivum (wheat), Zea mays (maize), and Lupinus angustifolius (lupin) were stained with either fat-soluble azo stain Sudan IV or fluorescent, diarylmethane Auramine O and were observed under confocal laser scanning microscope (CLSM). For all the plant species tested, the cuticle on the leaf surfaces could be clearly resolved in many cases into cuticular proper (CP), external cuticular layer (ECL), and internal cuticular layer (ICL). Novel image data analysis procedures for quantifying the epicuticular wax micromorphology were developed, and epicuticular waxes of L. angustifolius were described here for the first time. Together, application of a multifaceted approach involving the use of a range of techniques to study the plant cuticle has led to a better understanding of cuticular structure and provides new insights into leaf surface architecture.
Language eng
DOI 10.1007/s00709-015-0777-6
Field of Research 060702 Plant Cell and Molecular Biology
060705 Plant Physiology
070108 Sustainable Agricultural Development
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Grant ID ARCLP0991494
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30072099

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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