Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds

Tiwari,A, Tursky,ML, Kirkland,MA and Pande,G 2014, Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds, Current protocols in stem cell biology, vol. 28, pp. 1-1, doi: 10.1002/9780470151808.sc01c15s28.

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Title Expansion of human hematopoietic stem/progenitor cells on decellularized matrix scaffolds
Author(s) Tiwari,A
Journal name Current protocols in stem cell biology
Volume number 28
Start page 1
End page 1
Total pages 1
Publisher Wiley
Place of publication London, Eng.
Publication date 2014
ISSN 1938-8969
Keyword(s) decellularized biological scaffold
extracellular matrix
hematopoietic niche
stem cell expansion
umbilical cord blood
Antigens, CD34
Biological Markers
Cell Culture Techniques
Cell Proliferation
Cell Separation
Fetal Blood
Hematopoietic Stem Cells
Tissue Scaffolds
Summary Umbilical cord blood (UCB) is one of the richest sources for hematopoietic stem/progenitor cells (HSPCs), with more than 3000 transplantations performed each year for the treatment of leukemia and other bone marrow, immunological, and hereditary diseases. However, transplantation of single cord blood units is mostly restricted to children, due to the limited number of HSPC per unit. This unit develops a method to increase the number of HSPCs in laboratory conditions by using cell-free matrices from bone marrow cells that mimic 'human-body niche-like' conditions as biological scaffolds to support the ex vivo expansion of HSPCs. In this unit, we describe protocols for the isolation and characterization of HSPCs from UCB and their serum-free expansion on decellularized matrices. This method may also help to provide understanding of the biochemical organization of hematopoietic niches and lead to suggestions regarding the design of tissue engineering-based biomimetic scaffolds for HSPC expansion for clinical applications.
Language eng
DOI 10.1002/9780470151808.sc01c15s28
Field of Research 060106 Cellular Interactions (incl Adhesion, Matrix, Cell Wall)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Wiley-Blackwell
Persistent URL http://hdl.handle.net/10536/DRO/DU:30072475

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