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A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library.

Waugh,C, Cromer,D, Grimm,A, Chopra,A, Mallal,S, Davenport,M and Mak,J 2015, A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library., Virology Journal, vol. 12, no. 1, pp. 55, doi: 10.1186/s12985-015-0280-x.

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Title A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library.
Author(s) Waugh,C
Cromer,D
Grimm,A
Chopra,A
Mallal,S
Davenport,M
Mak,JORCID iD for Mak,J orcid.org/0000-0002-5229-5707
Journal name Virology Journal
Volume number 12
Issue number 1
Start page 55
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2015-04
ISSN 1743-422X
Summary Massive, parallel sequencing is a potent tool for dissecting the regulation of biological processes by revealing the dynamics of the cellular RNA profile under different conditions. Similarly, massive, parallel sequencing can be used to reveal the complexity of viral quasispecies that are often found in the RNA virus infected host. However, the production of cDNA libraries for next-generation sequencing (NGS) necessitates the reverse transcription of RNA into cDNA and the amplification of the cDNA template using PCR, which may introduce artefact in the form of phantom nucleic acids species that can bias the composition and interpretation of original RNA profiles.
Language eng
DOI 10.1186/s12985-015-0280-x
Field of Research 110804 Medical Virology
Socio Economic Objective 920109 Infectious Diseases
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID NHMRC Project Grant No 1025270
Copyright notice ©2015, BioMed Central
Persistent URL http://hdl.handle.net/10536/DRO/DU:30072620

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.