Peripheral injected cholecystokinin-8S modulates the concentration of serotonin in nerve fibers of the rat brainstem

Engster, Kim-Marie, Frommelt, Lisa, Hofmann, Tobias, Nolte, Sandra, Fischer, Felix, Rose, Matthias, Stengel, Andreas and Kobelt, Peter 2014, Peripheral injected cholecystokinin-8S modulates the concentration of serotonin in nerve fibers of the rat brainstem, Peptides, vol. 59, pp. 25-33, doi: 10.1016/j.peptides.2014.07.003.

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Title Peripheral injected cholecystokinin-8S modulates the concentration of serotonin in nerve fibers of the rat brainstem
Author(s) Engster, Kim-Marie
Frommelt, Lisa
Hofmann, Tobias
Nolte, SandraORCID iD for Nolte, Sandra
Fischer, Felix
Rose, Matthias
Stengel, Andreas
Kobelt, Peter
Journal name Peptides
Volume number 59
Start page 25
End page 33
Total pages 9
Publisher Elsevier Inc.
Place of publication Amsterdam, The Netherlands
Publication date 2014
ISSN 0196-9781
Keyword(s) C-Fos
Dorsal motor nucleus of the vagus
Nucleus of the solitary tract
Paraventricular nucleus of the hypothalamus
Summary Serotonin and cholecystokinin (CCK) play a role in the short-term inhibition of food intake. It is known that peripheral injection of CCK increases c-Fos-immunoreactivity (Fos-IR) in the nucleus of the solitary tract (NTS) in rats, and injection of the serotonin antagonist ondansetron decreases the number of c-Fos-IR cells in the NTS. This supports the idea of serotonin contributing to the effects of CCK. The aim of the present study was to elucidate whether peripherally injected CCK-8S modulates the concentration of serotonin in brain feeding-regulatory nuclei. Ad libitum fed male Sprague-Dawley rats received 5.2 and 8.7 nmol/kg CCK-8S (n = 3/group) or 0.15 M NaCl (n = 3-5/group) injected intraperitoneally (ip). The number of c-Fos-IR neurons, and the fluorescence intensity of serotonin in nerve fibers were assessed in the paraventricular nucleus (PVN), arcuate nucleus (ARC), NTS and dorsal motor nucleus of the vagus (DMV). CCK-8S increased the number of c-Fos-ir neurons in the NTS (mean ± SEM: 72 ± 4, and 112 ± 5 neurons/section, respectively) compared to vehicle-treated rats (7 ± 2 neurons/section, P < 0.05), but did not modulate c-Fos expression in the DMV or ARC. Additionally, CCK-8S dose-dependently increased the number of c-Fos-positive neurons in the PVN (218 ± 15 and 128 ± 14, respectively vs. 19 ± 5, P < 0.05). In the NTS and DMV we observed a decrease of serotonin-immunoreactivity 90 min after injection of CCK-8S (46 ± 2 and 49 ± 8 pixel/section, respectively) compared to vehicle (81 ± 8 pixel/section, P < 0.05). No changes of serotonin-immunoreactivity were observed in the PVN and ARC. Our results suggest that serotonin is involved in the mediation of CCK-8's effects in the brainstem. © 2014 Elsevier Inc.
Language eng
DOI 10.1016/j.peptides.2014.07.003
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2014, Elsevier
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Document type: Journal Article
Collections: Faculty of Health
School of Health and Social Development
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