Peripheral injected cholecystokinin-8S modulates the concentration of serotonin in nerve fibers of the rat brainstem

Engster, Kim-Marie, Frommelt, Lisa, Hofmann, Tobias, Nolte, Sandra, Fischer, Felix, Rose, Matthias, Stengel, Andreas and Kobelt, Peter 2014, Peripheral injected cholecystokinin-8S modulates the concentration of serotonin in nerve fibers of the rat brainstem, Peptides, vol. 59, pp. 25-33, doi: 10.1016/j.peptides.2014.07.003.

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Title Peripheral injected cholecystokinin-8S modulates the concentration of serotonin in nerve fibers of the rat brainstem
Author(s) Engster, Kim-Marie
Frommelt, Lisa
Hofmann, Tobias
Nolte, SandraORCID iD for Nolte, Sandra orcid.org/0000-0001-6185-9423
Fischer, Felix
Rose, Matthias
Stengel, Andreas
Kobelt, Peter
Journal name Peptides
Volume number 59
Start page 25
End page 33
Total pages 9
Publisher Elsevier Inc.
Place of publication Amsterdam, The Netherlands
Publication date 2014
ISSN 0196-9781
1873-5169
Keyword(s) C-Fos
Cholecystokinin
Dorsal motor nucleus of the vagus
Nucleus of the solitary tract
Paraventricular nucleus of the hypothalamus
Serotonin
Summary Serotonin and cholecystokinin (CCK) play a role in the short-term inhibition of food intake. It is known that peripheral injection of CCK increases c-Fos-immunoreactivity (Fos-IR) in the nucleus of the solitary tract (NTS) in rats, and injection of the serotonin antagonist ondansetron decreases the number of c-Fos-IR cells in the NTS. This supports the idea of serotonin contributing to the effects of CCK. The aim of the present study was to elucidate whether peripherally injected CCK-8S modulates the concentration of serotonin in brain feeding-regulatory nuclei. Ad libitum fed male Sprague-Dawley rats received 5.2 and 8.7 nmol/kg CCK-8S (n = 3/group) or 0.15 M NaCl (n = 3-5/group) injected intraperitoneally (ip). The number of c-Fos-IR neurons, and the fluorescence intensity of serotonin in nerve fibers were assessed in the paraventricular nucleus (PVN), arcuate nucleus (ARC), NTS and dorsal motor nucleus of the vagus (DMV). CCK-8S increased the number of c-Fos-ir neurons in the NTS (mean ± SEM: 72 ± 4, and 112 ± 5 neurons/section, respectively) compared to vehicle-treated rats (7 ± 2 neurons/section, P < 0.05), but did not modulate c-Fos expression in the DMV or ARC. Additionally, CCK-8S dose-dependently increased the number of c-Fos-positive neurons in the PVN (218 ± 15 and 128 ± 14, respectively vs. 19 ± 5, P < 0.05). In the NTS and DMV we observed a decrease of serotonin-immunoreactivity 90 min after injection of CCK-8S (46 ± 2 and 49 ± 8 pixel/section, respectively) compared to vehicle (81 ± 8 pixel/section, P < 0.05). No changes of serotonin-immunoreactivity were observed in the PVN and ARC. Our results suggest that serotonin is involved in the mediation of CCK-8's effects in the brainstem. © 2014 Elsevier Inc.
Language eng
DOI 10.1016/j.peptides.2014.07.003
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2014, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30073101

Document type: Journal Article
Collection: School of Health and Social Development
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