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Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training

Vincent, Grace, Lamon, Severine, Gant, Nicholas, Vincent, Peter J., MacDonald, Julia R., Markworth, James F., Edge, Johann A. and Hickey, Anthony J. 2015, Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training, Frontiers in Physiology, vol. 6, Article no: 51, pp. 1-8, doi: 10.3389/fphys.2015.00051.

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Title Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training
Author(s) Vincent, Grace
Lamon, SeverineORCID iD for Lamon, Severine orcid.org/0000-0002-3271-6551
Gant, Nicholas
Vincent, Peter J.
MacDonald, Julia R.
Markworth, James F.
Edge, Johann A.
Hickey, Anthony J.
Journal name Frontiers in Physiology
Volume number 6
Season Article no: 51
Start page 1
End page 8
Total pages 8
Publisher Frontiers
Place of publication Lausanne, Switzerland
Publication date 2015-02-24
ISSN 1664-042X
Keyword(s) HIT
PGC-1α
mitochondria
oxidative phosphorylation
skeletal muscle
Science & Technology
Life Sciences & Biomedicine
Physiology
PGC-1 alpha
HUMAN SKELETAL-MUSCLE
TERM SPRINT INTERVAL
LOW-VOLUME
METABOLIC ADAPTATIONS
TRANSCRIPTION FACTORS
EXERCISE PERFORMANCE
ENERGY-METABOLISM
CROSS-EDUCATION
INCREASES
BIOGENESIS
Summary PURPOSE: High-intensity short-duration interval training (HIT) stimulates functional and metabolic adaptation in skeletal muscle, but the influence of HIT on mitochondrial function remains poorly studied in humans. Mitochondrial metabolism as well as mitochondrial-associated protein expression were tested in untrained participants performing HIT over a 2-week period. METHODS: Eight males performed a single-leg cycling protocol (12 × 1 min intervals at 120% peak power output, 90 s recovery, 4 days/week). Muscle biopsies (vastus lateralis) were taken pre- and post-HIT. Mitochondrial respiration in permeabilized fibers, citrate synthase (CS) activity and protein expression of peroxisome proliferator-activated receptor gamma coactivator (PGC-1α) and respiratory complex components were measured. RESULTS: HIT training improved peak power and time to fatigue. Increases in absolute oxidative phosphorylation (OXPHOS) capacities and CS activity were observed, but not in the ratio of CCO to the electron transport system (CCO/ETS), the respiratory control ratios (RCR-1 and RCR-2) or mitochondrial-associated protein expression. Specific increases in OXPHOS flux were not apparent after normalization to CS, indicating that gross changes mainly resulted from increased mitochondrial mass. CONCLUSION: Over only 2 weeks HIT significantly increased mitochondrial function in skeletal muscle independently of detectable changes in mitochondrial-associated and mitogenic protein expression.
Language eng
DOI 10.3389/fphys.2015.00051
Field of Research 110699 Human Movement and Sports Science not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Frontiers
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30073452

Document type: Journal Article
Collections: School of Exercise and Nutrition Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.