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The inhibitory effect of stevioside on bacillus cereus growth in milk: validation and its response surface optimization

Sharma, Deepika and Puri, Munish 2015, The inhibitory effect of stevioside on bacillus cereus growth in milk: validation and its response surface optimization, Current biotechnology, vol. 4, no. 1, pp. 56-64, doi: 10.2174/2211550104666150423000314#sthash.630aV6Jg.dpuf.

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Title The inhibitory effect of stevioside on bacillus cereus growth in milk: validation and its response surface optimization
Author(s) Sharma, Deepika
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Current biotechnology
Volume number 4
Issue number 1
Start page 56
End page 64
Total pages 9
Publisher Bentham Science Publishers
Place of publication Bussum, The Netherlands
Publication date 2015
ISSN 2211-5501
2211-551X
Keyword(s) Antimicrobial compounds
Bioactives
Food poisoning
Response surface methodology
Summary Stevioside, a glycoside present in the leaves of Stevia rebaudiana Bertoni, offers therapeutic benefits such as anti-hyperglycemic, anti-hypertensive, antiinflammatory, anti-tumor, diuretic and immune influencing properties. In this work antimicrobial activity of stevioside against Bacillus cereus, a major source of milk contamination was investigated. The isolate was confirmed by various biochemical and 16S rRNA gene sequencing. The effect of temperature, incubation time and concentration of stevioside was optimized from a central composite response surface design. The standard plate count (SPC) of pasteurized milk was drastically reduced in comparison to toned and fresh milk. The optimal temperature, incubation time and stevioside concentration were observed to be 60.23°C, 21 h, and 275 μg/ mL respectively. The synergism of stevioside with the external factors (temperature and time) against B. cereus was observed. Our studies showed that addition of stevioside in fresh as well as pasteurised milk would control growth of B. cereus in milk.
Language eng
DOI 10.2174/2211550104666150423000314#sthash.630aV6Jg.dpuf
Field of Research 060501 Bacteriology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Bentham Science Publishers
Persistent URL http://hdl.handle.net/10536/DRO/DU:30073527

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