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Regulation of HbPIP2;3, a latex-abundant water transporter, is associated with latex dilution and yield in the rubber tree (Hevea brasiliensis Muell. Arg.)

An, Feng, Zou, Zhi, Cai, Xiuqing, Wang, Jin, Rookes, James, Lin, Weifu, Cahill, David and Kong, Lingxue 2015, Regulation of HbPIP2;3, a latex-abundant water transporter, is associated with latex dilution and yield in the rubber tree (Hevea brasiliensis Muell. Arg.), PLoS one, vol. 10, no. 4, Article Number : e0125595, pp. 1-21, doi: 10.1371/journal.pone.0125595.

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Title Regulation of HbPIP2;3, a latex-abundant water transporter, is associated with latex dilution and yield in the rubber tree (Hevea brasiliensis Muell. Arg.)
Author(s) An, Feng
Zou, Zhi
Cai, Xiuqing
Wang, Jin
Rookes, JamesORCID iD for Rookes, James orcid.org/0000-0003-1479-2339
Lin, Weifu
Cahill, DavidORCID iD for Cahill, David orcid.org/0000-0002-2556-0528
Kong, LingxueORCID iD for Kong, Lingxue orcid.org/0000-0001-6219-3897
Journal name PLoS one
Volume number 10
Issue number 4
Season Article Number : e0125595
Start page 1
End page 21
Total pages 21
Publisher PLOS
Place of publication San Francisco, Calif.
Publication date 2015-04-30
ISSN 1932-6203
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
PLANT AQUAPORINS
FUNCTIONAL-CHARACTERIZATION
ETHYLENE STIMULATION
CHANNEL ACTIVITY
EXPRESSION
GENE
SEQUENCE
DROUGHT
PROTEIN
BALANCE
Summary Rubber tree (Hevea brasiliensis) latex, the source of natural rubber, is synthesised in the cytoplasm of laticifers. Efficient water inflow into laticifers is crucial for latex flow and production since it is the determinant of the total solid content of latex and its fluidity after tapping. As the mature laticifer vessel rings are devoid of plasmodesmata, water exchange between laticifers and surrounding cells is believed to be governed by plasma membrane intrinsic proteins (PIPs). To identify the most important PIP aquaporin in the water balance of laticifers, the transcriptional profiles of ten-latex-expressed PIPs were analysed. One of the most abundant transcripts, designated HbPIP2;3, was characterised in this study. When tested in Xenopus laevis oocytes HbPIP2;3 showed a high efficiency in increasing plasmalemma water conductance. Expression analysis indicated that the HbPIP2;3 gene was preferentially expressed in latex, and the transcripts were up-regulated by both wounding and exogenously applied Ethrel (a commonly-used ethylene releaser). Although regular tapping up-regulated the expression of HbPIP2;3 during the first few tappings of the virginal rubber trees, the transcriptional kinetics of HbPIP2;3 to Ethrel stimulation in the regularly tapped tree exhibited a similar pattern to that of the previously reported HbPIP2;1 in the virginal rubber trees. Furthermore, the mRNA level of HbPIP2;3 was associated with clonal yield potential and the Ethrel stimulation response. Together, these results have revealed the central regulatory role of HbPIP2;3 in laticifer water balance and ethylene stimulation of latex production in Hevea.
Notes Reproduced with the kind permission of the copyright owner.
Language eng
DOI 10.1371/journal.pone.0125595
Field of Research 060705 Plant Physiology
060405 Gene Expression (incl Microarray and other genome-wide approaches)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30073825

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.