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Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction

Agyei, Dominic, Lim, Wendy, Zass, Michael, Tan, Darren and Danquah, Michael K. 2013, Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction, Chemical engineering science, vol. 95, pp. 323-330, doi: 10.1016/j.ces.2013.03.049.

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Title Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction
Formatted title Bioanalytical evaluation of Lactobacillus delbrueckii subsp. lactis 313 cell-envelope proteinase extraction
Author(s) Agyei, DominicORCID iD for Agyei, Dominic orcid.org/0000-0003-2280-4096
Lim, Wendy
Zass, Michael
Tan, Darren
Danquah, Michael K.
Journal name Chemical engineering science
Volume number 95
Start page 323
End page 330
Total pages 8
Publisher Elsevier
Place of publication Oxford, Eng.
Publication date 2013-05-24
ISSN 0009-2509
1873-4405
Keyword(s) cell-envelope proteinases
proteolytic system
Lactobacillus delbrueckii subsp. lactis 313
cell wall binding
enzyme extraction
Summary Lactobacilli cell-envelope proteinases (CEPs) have demonstrated numerous biopharmaceutical applications in the development of new streams of blockbuster nutraceuticals; thus, the development of efficient and commercially viable methods for CEP extraction will promote their full-scale application. In this study, the sub-cellular location of CEPs in Lactobacillus delbrueckii subsp. lactis 313 (LDL 313) was identified and the effects of different extraction methods were investigated for their ability to efficiently release CEPs from LDL 313. Significantly high relative proteinase activity of~95% was detected in cell-wall fractions and ~5% activity was observed for osmotic fluids, implying that proteinases in LDL 313 are cell-wall bound. CEPs were released from cell-wall via incubation in calcium-free buffer, indicating the enzyme is liable to self-digestion and ionic misfolding. Of the different extraction methods investigated, the use of 5 M LiCl was the most suitable, under the conditions of experimentation, for releasing high levels of CEPs from LDL 313.
Language eng
DOI 10.1016/j.ces.2013.03.049
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
100303 Fermentation
Socio Economic Objective 860899 Human Pharmaceutical Products not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30074187

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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