Understanding physicochemical changes in pretreated and enzyme hydrolysed hemp (Cannabis sativa) biomass for biorefinery development

Abraham, Reinu, Vongsvivut, Jitraporn, Barrow, Colin J. and Puri, Munish 2016, Understanding physicochemical changes in pretreated and enzyme hydrolysed hemp (Cannabis sativa) biomass for biorefinery development, Biomass conversion and biorefinery, vol. 6, no. 2, pp. 127-138, doi: 10.1007/s13399-015-0168-4.

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Title Understanding physicochemical changes in pretreated and enzyme hydrolysed hemp (Cannabis sativa) biomass for biorefinery development
Author(s) Abraham, Reinu
Vongsvivut, Jitraporn
Barrow, Colin J.ORCID iD for Barrow, Colin J. orcid.org/0000-0002-2153-7267
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Biomass conversion and biorefinery
Volume number 6
Issue number 2
Start page 127
End page 138
Total pages 12
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-06
ISSN 2190-6815
2190-6823
Keyword(s) Feedstock
Crystallinity index
Cellulose
Biofuel
FTIR
x-ray
NMR
Summary The physicochemical properties of hemp biomass structure to pretreatment and enzymatic hydrolysis were investigated to improve upon reducing sugar production for biofuel development. Sodium hydroxide pretreated biomass (SHPB) yielded maximum conversion of holocellulose into reducing sugar (72 %). Scanning electron microscopy (SEM) revealed that enzymatic hydrolysis generated regular micropores in the fragmented biomass structure. The thermogravimetric analysis (TGA) curve suggested the degradation of hemicellulose and cellulose, which conformed well to the subsequent nuclear magnetic resonance (NMR) studies indicating the presence of α- and β-glucose (28.4 %) and α- and β-xylose (10.7 %), the major carbohydrate components commonly found in hydrolysis products of hemicellulose and cellulose. Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectra showed stretching modes of the lignin acetyl group, suggesting the loosening of the polymer matrix and thus the exposure of the cellulose polymorphs. X-ray diffraction pattern indicated that enzymatic hydrolysis caused a higher crystallinity index (36.71), due to the fragmentation of amorphous cellulose leading to the reducing sugar production suitable for biofuel development.
Language eng
DOI 10.1007/s13399-015-0168-4
Field of Research 060101 Analytical Biochemistry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30074611

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