Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection

Pandohee, Jessica, Holland, Brendan J., Li, Bingshan, Tsuzuki, Takuya, Stevenson, Paul G., Barnett, Neil W., Pearson, James R., Jones, Oliver A. H. and Conlan, Xavier A. 2015, Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection, Journal of separation science, vol. 38, no. 12, pp. 2024-2032, doi: 10.1002/jssc.201500088.

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Title Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection
Author(s) Pandohee, Jessica
Holland, Brendan J.
Li, Bingshan
Tsuzuki, Takuya
Stevenson, Paul G.ORCID iD for Stevenson, Paul G. orcid.org/0000-0001-6780-6859
Barnett, Neil W.
Pearson, James R.
Jones, Oliver A. H.
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Journal name Journal of separation science
Volume number 38
Issue number 12
Start page 2024
End page 2032
Total pages 9
Publisher Wiley
Place of publication Berlin, Germany
Publication date 2015-06
ISSN 1615-9314
Keyword(s) 2D chromatography
Cannabis
Offline chromatography
Profiling
Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
MASS-SPECTROMETRY
SATIVA L
CONSTITUENTS
SEPARATIONS
ANTIOXIDANT
PRODUCTS
SOLVENT
CELL
Summary Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.
Language eng
DOI 10.1002/jssc.201500088
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Wiley
Persistent URL http://hdl.handle.net/10536/DRO/DU:30074737

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