Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Bhardwaj, Nandana, Rajkhowa, Rangam, Wang, Xungai and Devi, Dipali 2015, Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications, International journal of biological macromolecules, vol. 81, pp. 31-40, doi: 10.1016/j.ijbiomac.2015.07.049.

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Title Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications
Author(s) Bhardwaj, Nandana
Rajkhowa, RangamORCID iD for Rajkhowa, Rangam
Wang, XungaiORCID iD for Wang, Xungai
Devi, Dipali
Journal name International journal of biological macromolecules
Volume number 81
Start page 31
End page 40
Total pages 10
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-07-29
ISSN 0141-8130
Keyword(s) Biomaterials
Biomedical applications
Drug delivery
Non-mulberry silk
Silk fibroin
Summary Silk fibroin has been widely employed in various forms as biomaterials for biomedical applications due to its superb biocompatibility and tunable degradation and mechanical properties. Herein, silk fibroin microparticles of non-mulberry silkworm species (Antheraea assamensis, Antheraea mylitta and Philosamia ricini) were fabricated via a top-down approach using a combination of wet-milling and spray drying techniques. Microparticles of mulberry silkworm (Bombyx mori) were also utilized for comparative studies. The fabricated microparticles were physico-chemically characterized for size, stability, morphology, chemical composition and thermal properties. The silk fibroin microparticles of all species were porous (∼5μm in size) and showed nearly spherical morphology with rough surface as revealed from dynamic light scattering and microscopic studies. Non-mulberry silk microparticles maintained the typical silk-II structure with β-sheet secondary conformation with higher thermal stability. Additionally, non-mulberry silk fibroin microparticles supported enhanced cell adhesion, spreading and viability of mouse fibroblasts than mulberry silk fibroin microparticles (p<0.001) as evidenced from fluorescence microscopy and cytotoxicity studies. Furthermore, in vitro drug release from the microparticles showed a significantly sustained release over 3 weeks. Taken together, this study demonstrates promising attributes of non-mulberry silk fibroin microparticles as a potential drug delivery vehicle/micro carrier for diverse biomedical applications.
Language eng
DOI 10.1016/j.ijbiomac.2015.07.049
Field of Research 090301 Biomaterials
Socio Economic Objective 860403 Natural Fibres, Yarns and Fabrics
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Elsevier
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Document type: Journal Article
Collections: Institute for Frontier Materials
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