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Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography

Bassanese, Danielle N., Conlan, Xavier A., Barnett, Neil W. and Stevenson, Paul G. 2015, Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography, Journal separation science, vol. 38, no. 10, pp. 1642-1648, doi: 10.1002/jssc.201500054.

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Title Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography
Author(s) Bassanese, Danielle N.
Conlan, Xavier A.ORCID iD for Conlan, Xavier A. orcid.org/0000-0003-0829-0551
Barnett, Neil W.
Stevenson, Paul G.ORCID iD for Stevenson, Paul G. orcid.org/0000-0001-6780-6859
Journal name Journal separation science
Volume number 38
Issue number 10
Start page 1642
End page 1648
Total pages 7
Publisher Wiley
Place of publication London, Eng.
Publication date 2015-05
ISSN 1615-9314
Keyword(s) Antioxidants
Countergradient
Limit of detection
Solvent strength mismatch
Two-dimensional high-performance liquid chromatography
Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
TANDEM MASS-SPECTROMETRY
DIODE-ARRAY DATA
STEVIA-REBAUDIANA
MOBILE-PHASE
QUANTIFICATION
PROTEOMICS
INJECTION
PEAKS
2ND-DIMENSION
COLUMNS
Summary This paper explores the analytical figures of merit of two-dimensional high-performance liquid chromatography for the separation of antioxidant standards. The cumulative two-dimensional high-performance liquid chromatography peak area was calculated for 11 antioxidants by two different methods--the areas reported by the control software and by fitting the data with a Gaussian model; these methods were evaluated for precision and sensitivity. Both methods demonstrated excellent precision in regards to retention time in the second dimension (%RSD below 1.16%) and cumulative second dimension peak area (%RSD below 3.73% from the instrument software and 5.87% for the Gaussian method). Combining areas reported by the high-performance liquid chromatographic control software displayed superior limits of detection, in the order of 1 × 10(-6) M, almost an order of magnitude lower than the Gaussian method for some analytes. The introduction of the countergradient eliminated the strong solvent mismatch between dimensions, leading to a much improved peak shape and better detection limits for quantification.
Language eng
DOI 10.1002/jssc.201500054
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID DP140100439
RM26626
Copyright notice ©2015, Wiley
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075077

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