Development of a resin based silica monolithic column encapsulation
Spilstead, Kara B., Haswell, Stephen J., Barnett, Neil W., Conlan, Xavier A., Stevenson, Paul G. and Francis, Paul S. 2015, Development of a resin based silica monolithic column encapsulation, Analytical methods, vol. 7, no. 12, pp. 4908-4911, doi: 10.1039/c5ay00722d.
As monolithic columns become more extensively used in separation based applications due to their good flow and high surface characteristics, there has arisen the need to establish simple, reliable fabrication methods for fluidic coupling and sealing. In particular, the problem of liquid tracking between a monolith's outer surface and the sealing wall, resulting in poor flow-through performance, needs to be addressed. This paper describes a novel resin-based encapsulation method that penetrates 0.3 mm into the outer surface of a 4 mm diameter monolith, removing the so-called wall-effect. Results based on the peak analysis from 1 μL of 0.4% thiourea injected into a 98:2 water:methanol mobile phase flowing at 1 mL min-1 indicate excellent flow conservation through the monolith. A comparison of peak shape and height equivalent to a theoretical plate (HETP) data between the reported resin-based method and the previously reported heat shrink tubing encapsulation methodology, for the same batch of monoliths, suggests the resin based method offers far superior flow characteristics. In addition to the improved flow properties, the resin casting method enables standard polyether ether ketone (PEEK) fittings to be moulded and subsequently unscrewed from the device offering simple reliable fluidic coupling to be achieved.
Language
eng
DOI
10.1039/c5ay00722d
Field of Research
030108 Separation Science 0301 Analytical Chemistry 0399 Other Chemical Sciences
Socio Economic Objective
970103 Expanding Knowledge in the Chemical Sciences
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