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Maternal creatine homeostasis is altered during gestation in the spiny mouse: is this a metabolic adaptation to pregnancy?

Ellery, Stacey J., LaRosa, Domenic A., Kett, Michelle M., Della Gatta, Paul A., Snow, Rod J., Walker, David W. and Dickinson, Hayley 2015, Maternal creatine homeostasis is altered during gestation in the spiny mouse: is this a metabolic adaptation to pregnancy?, BMC pregnancy childbirth, vol. 15, no. 92, pp. 1-9, doi: 10.1186/s12884-015-0524-1.

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Title Maternal creatine homeostasis is altered during gestation in the spiny mouse: is this a metabolic adaptation to pregnancy?
Author(s) Ellery, Stacey J.
LaRosa, Domenic A.
Kett, Michelle M.
Della Gatta, Paul A.ORCID iD for Della Gatta, Paul A. orcid.org/0000-0003-2231-8370
Snow, Rod J.ORCID iD for Snow, Rod J. orcid.org/0000-0002-4796-6916
Walker, David W.
Dickinson, Hayley
Journal name BMC pregnancy childbirth
Volume number 15
Issue number 92
Start page 1
End page 9
Total pages 9
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2015
ISSN 1471-2393
Keyword(s) Cellular energy
Fetal development
Metabolism
Nutrition
Pregnancy
Spiny mouse
Science & Technology
Life Sciences & Biomedicine
Obstetrics & Gynecology
X-RAY ABSORPTIOMETRY
SKELETAL-MUSCLE MASS
GASTROINTESTINAL MOTILITY
ENERGY-REQUIREMENTS
TRANSPORTER
EXPRESSION
SUPPLEMENTATION
LIVER
BIRTH
LACTATION
Summary BACKGROUND: Pregnancy induces adaptations in maternal metabolism to meet the increased need for nutrients by the placenta and fetus. Creatine is an important intracellular metabolite obtained from the diet and also synthesised endogenously. Experimental evidence suggests that the fetus relies on a maternal supply of creatine for much of gestation. However, the impact of pregnancy on maternal creatine homeostasis is unclear. We hypothesise that alteration of maternal creatine homeostasis occurs during pregnancy to ensure adequate levels of this essential substrate are available for maternal tissues, the placenta and fetus. This study aimed to describe maternal creatine homeostasis from mid to late gestation in the precocial spiny mouse. METHODS: Plasma creatine concentration and urinary excretion were measured from mid to late gestation in pregnant (n = 8) and age-matched virgin female spiny mice (n = 6). At term, body composition and organ weights were assessed and tissue total creatine content determined. mRNA expression of the creatine synthesising enzymes arginine:glycine amidinotransferase (AGAT) and guanidinoacetate methyltransferase (GAMT), and the creatine transporter (CrT1) were assessed by RT-qPCR. Protein expression of AGAT and GAMT was also assessed by western blot analysis. RESULTS: Plasma creatine and renal creatine excretion decreased significantly from mid to late gestation (P < 0.001, P < 0.05, respectively). Pregnancy resulted in increased lean tissue (P < 0.01), kidney (P < 0.01), liver (P < 0.01) and heart (P < 0.05) mass at term. CrT1 expression was increased in the heart (P < 0.05) and skeletal muscle (P < 0.05) at term compared to non-pregnant tissues, and creatine content of the heart (P < 0.05) and kidney (P < 0.001) were also increased at this time. CrT1 mRNA expression was down-regulated in the liver (<0.01) and brain (<0.01) of pregnant spiny mice at term. Renal AGAT mRNA (P < 0.01) and protein (P < 0.05) expression were both significantly up-regulated at term, with decreased expression of AGAT mRNA (<0.01) and GAMT protein (<0.05) observed in the term pregnant heart. Brain AGAT (<0.01) and GAMT (<0.001) mRNA expression were also decreased at term. CONCLUSION: Change of maternal creatine status (increased creatine synthesis and reduced creatine excretion) may be a necessary adjustment of maternal physiology to pregnancy to meet the metabolic demands of maternal tissues, the placenta and developing fetus.
Language eng
DOI 10.1186/s12884-015-0524-1
Field of Research 090899 Food Sciences not elsewhere classified
119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, BioMed Central
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075143

Document type: Journal Article
Collections: School of Exercise and Nutrition Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.