Vasodilatory effects of homologous adrenomedullin 2 and adrenomedullin 5 on isolated blood vessels of two species of eel

Cameron, Melissa S., Nobata, Shigenori, Takei, Yoshio and Donald, John A. 2015, Vasodilatory effects of homologous adrenomedullin 2 and adrenomedullin 5 on isolated blood vessels of two species of eel, Comparative biochemistry and physiology part a: molecular and integrative physiology, vol. 179, pp. 157-163, doi: 10.1016/j.cbpa.2014.09.034.

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Title Vasodilatory effects of homologous adrenomedullin 2 and adrenomedullin 5 on isolated blood vessels of two species of eel
Author(s) Cameron, Melissa S.
Nobata, Shigenori
Takei, Yoshio
Donald, John A.ORCID iD for Donald, John A. orcid.org/0000-0001-5930-2642
Journal name Comparative biochemistry and physiology part a: molecular and integrative physiology
Volume number 179
Start page 157
End page 163
Total pages 7
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-01
ISSN 1531-4332
Keyword(s) Adrenomedullin
Anguilla
Endothelium
Nitric oxide
Teleost
Vasodilation
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Physiology
Zoology
GENE-RELATED PEPTIDE
ACTIVATED POTASSIUM CHANNELS
NITRIC-OXIDE
CARDIOVASCULAR ACTIONS
VASCULAR BED
RAT
FAMILY
RECEPTORS
INTERMEDIN/ADRENOMEDULLIN-2
MAMMALS
Summary In mammals, adrenomedullin (AM) is a potent vasodilator through signalling pathways that involve the endothelium. In teleost fishes, a family of five AMs are present (AM1/4, AM2/3 and AM5) with four homologous AMs (AM1, AM2/3 and AM5) recently cloned from the Japanese eel, Anguilla japonica. Both AM2 and AM5 have been shown to be strong in vivo vasodepressors in eel, but the mechanism of action of homologous AMs on isolated blood vessels has not been examined in teleost fish. In this study, both eel AM2 and AM5 caused a marked vasodilation of the dorsal aorta. However, only AM5 consistently dilated the small gonadal artery in contrast to AM2 that had no effect in most preparations. Neither AM2 nor AM5 had any effect when applied to the first afferent branchial artery; in contrast, eel ANP always caused a large vasodilation of the branchial artery. In the dorsal aorta, indomethacin significantly reduced the AM2 vasodilation, but had no effect on the AM5 vasodilation. In contrast, removal of the endothelium significantly enhanced the AM5 vasodilation only. In the gonadal artery, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one (ODQ) significantly reduced the AM5 vasodilation suggesting a role for soluble guanylyl cyclase in the dilation, but l-NNA and removal of the endothelium had no effect. The results of this study indicate that AM2 and AM5 have distinct vasodilatory effects that may be due to the peptides signalling via different receptors to regulate vascular tone in eel.
Language eng
DOI 10.1016/j.cbpa.2014.09.034
Field of Research 060807 Animal Structure and Function
0601 Biochemistry And Cell Biology
0606 Physiology
0608 Zoology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075282

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