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Nutrient provision increases signalling and protein synthesis in human skeletal muscle after repeated sprints

Coffey, Vernon G, Moore, Daniel R, Burd, Nicholas A, Rerecich, Tracy, Stellingwerff, Trent, Garnham, Andrew P, Phillips, Stuart M and Hawley, John A 2011, Nutrient provision increases signalling and protein synthesis in human skeletal muscle after repeated sprints, European journal of applied physiology, vol. 111, no. 7, pp. 1473-1483, doi: 10.1007/s00421-010-1768-0.

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Title Nutrient provision increases signalling and protein synthesis in human skeletal muscle after repeated sprints
Author(s) Coffey, Vernon G
Moore, Daniel R
Burd, Nicholas A
Rerecich, Tracy
Stellingwerff, Trent
Garnham, Andrew P
Phillips, Stuart M
Hawley, John A
Journal name European journal of applied physiology
Volume number 111
Issue number 7
Start page 1473
End page 1483
Total pages 11
Publisher Springer Verlag
Place of publication Berlin, Germany
Publication date 2011-07
ISSN 1439-6319
1439-6327
Keyword(s) Acceleration
Adult
Cross-Over Studies
Double-Blind Method
Eating
Food
Humans
Male
Muscle, Skeletal
Periodicity
Placebos
Protein Biosynthesis
Running
Signal Transduction
Up-Regulation
Young Adult
Science & Technology
Life Sciences & Biomedicine
Physiology
Sport Sciences
Exercise
Fractional synthetic rate
Cell signalling
Summary The effect of nutrient availability on the acute molecular responses following repeated sprint exercise is unknown. The aim of this study was to determine skeletal muscle cellular and protein synthetic responses following repeated sprint exercise with nutrient provision. Eight healthy young male subjects undertook two sprint cycling sessions (10 × 6 s, 0.75 N m torque kg(-1), 54 s recovery) with either pre-exercise nutrient (24 g whey, 4.8 g leucine, 50 g maltodextrin) or non-caloric placebo ingestion. Muscle biopsies were taken from vastus lateralis at rest, and after 15 and 240 min post-exercise recovery to determine muscle cell signalling responses and protein synthesis by primed constant infusion of L: -[ring-(13)C(6)] phenylalanine. Peak and mean power outputs were similar between nutrient and placebo trials. Post-exercise myofibrillar protein synthetic rate was greater with nutrient ingestion compared with placebo (~48%, P < 0.05) but the rate of mitochondrial protein synthesis was similar between treatments. The increased myofibrillar protein synthesis following sprints with nutrient ingestion was associated with coordinated increases in Akt-mTOR-S6K-rpS6 phosphorylation 15 min post-exercise (~200-600%, P < 0.05), while there was no effect on these signalling molecules when exercise was undertaken in the fasted state. For the first time we report a beneficial effect of nutrient provision on anabolic signalling and muscle myofibrillar protein synthesis following repeated sprint exercise. Ingestion of protein/carbohydrate in close proximity to high-intensity sprint exercise provides an environment that increases cell signalling and protein synthesis.
Language eng
DOI 10.1007/s00421-010-1768-0
Field of Research 110604 Sports Medicine
1106 Human Movement And Sports Science
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2010, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075452

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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