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Activation of mTOR signalling in young and old human skeletal muscle in response to combined resistance exercise and whey protein ingestion

Farnfield, Michelle M., Breen, Leigh, Carey, Kate A., Garnham, Andrew and Cameron-Smith, David 2012, Activation of mTOR signalling in young and old human skeletal muscle in response to combined resistance exercise and whey protein ingestion, Applied physiology, nutrition, and metabolism, vol. 37, no. 1, pp. 21-30, doi: 10.1139/h11-132.

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Title Activation of mTOR signalling in young and old human skeletal muscle in response to combined resistance exercise and whey protein ingestion
Author(s) Farnfield, Michelle M.
Breen, Leigh
Carey, Kate A.
Garnham, Andrew
Cameron-Smith, David
Journal name Applied physiology, nutrition, and metabolism
Volume number 37
Issue number 1
Start page 21
End page 30
Total pages 10
Publisher NRC Research Press
Place of publication Ottawa, Ont.
Publication date 2012-02
ISSN 1715-5312
1715-5320
Keyword(s) resistance exercise training
amino acids
translation initiation
age
Adaptor Proteins, Signal Transducing
Adolescent
Adult
Age Factors
Aged
Aging
Analysis of Variance
Biopsy
Enzyme Activation
Eukaryotic Initiation Factor-4G
Humans
Hypertrophy
Male
Middle Aged
Milk Proteins
Muscle Strength
Phosphoproteins
Phosphorylation
Quadriceps Muscle
Resistance Training
Ribosomal Protein S6 Kinases, 70-kDa
Signal Transduction
TOR Serine-Threonine Kinases
Time Factors
Victoria
Whey Proteins
Young Adult
Science & Technology
Life Sciences & Biomedicine
Nutrition & Dietetics
Physiology
Sport Sciences
ESSENTIAL AMINO-ACIDS
ELDERLY HUMANS
DIFFERENTIAL STIMULATION
ANABOLIC RESPONSE
DOSE-RESPONSE
ENHANCES MTOR
MEN
MYOFIBRILLAR
Summary PURPOSE: To investigate the impact of whey protein ingestion and resistance exercise training on the phosphorylation of mRNA translational signalling proteins in the skeletal muscle of young and old men.

METHODS: Sixteen healthy young (aged 18-25 years) and 15 healthy older men (aged 60-75 years) completed 12 weeks of resistance exercise and were randomly assigned to consume a whey protein (WPI) or placebo drink after each session. Muscle biopsies were collected before and 2 h after an acute exercise bout at the beginning and the end of training.

RESULTS: All subjects significantly increased strength after following strength training. Phosphorylation of mTOR was significantly greater in the WPI groups compared with placebo for both younger and older subjects. Phosphorylation of p70S6K, eIF4G, and 4EBP1 was greater for older subjects consuming WPI. Phosphorylation of rpS6, eIF4G, and 4EBP1 tended to increase in the younger subjects that had consumed WPI. Post-training, younger subjects demonstrated a similar pattern of mTOR phosphorylation as seen pre-training. In contrast, the initial heightened phosphorylation of mTOR, p70S6K, rpS6, and eIF4G in older muscle to combined resistance exercise and WPI ingestion became less pronounced after repeated training sessions.

CONCLUSIONS: In the untrained state, resistance exercise coupled with WPI increases the phosphorylation of proteins involved in mRNA translation compared with exercise alone. Post-training, WPI- and exercise-induced protein phosphorylation was reduced in older men, but not in younger men. Thus, strategies to induce hypertrophy should utilize protein and resistance training concurrently. Further investigations should delineate interventions that will maintain sensitivity to anabolic stimuli in older populations.
Language eng
DOI 10.1139/h11-132
Field of Research 110699 Human Movement and Sports Science not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, Canadian Science Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075453

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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