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Evaluation of the MAIIA dipstick test to detect recombinant human erythropoietin in plasma

Ashenden, Michael, Sharpe, Ken, Garnham, Andrew and Gore, Christopher J. 2012, Evaluation of the MAIIA dipstick test to detect recombinant human erythropoietin in plasma, Journal of pharmaceutical and biomedical analysis, vol. 67-68, pp. 123-128, doi: 10.1016/j.jpba.2012.04.025.

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Title Evaluation of the MAIIA dipstick test to detect recombinant human erythropoietin in plasma
Author(s) Ashenden, Michael
Sharpe, Ken
Garnham, Andrew
Gore, Christopher J.
Journal name Journal of pharmaceutical and biomedical analysis
Volume number 67-68
Start page 123
End page 128
Total pages 6
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2012-08
ISSN 0731-7085
1873-264X
Keyword(s) blood doping
recombinant human erythropoietin
MAIIA
Adult
Erythropoietin
Humans
Limit of Detection
Male
Recombinant Proteins
Science & Technology
Physical Sciences
Life Sciences & Biomedicine
Chemistry, Analytical
Pharmacology & Pharmacy
Chemistry
ISOELECTRIC PROFILES
URINE
SERUM
Summary Recently a novel technology, referred to as the 'EPO WGA MAIIA' test, has been developed by Swedish researchers to discriminate between endogenous and recombinant human erythropoietin. In contrast to existing electrophoretic methods that are used by antidoping laboratories, this dipstick-based technique is simple and fast. Moreover it can be applied to either blood or urine specimens. These characteristics could prove advantageous if the test were adopted by antidoping authorities to determine blood doping in sport.

We evaluated the sensitivity of EPO WGA MAIIA to detect the presence of recombinant human erythropoietin (rhEPO) in some archived plasma specimens which had been collected from healthy, active subjects either 72 h or 96 h after a 'microdose' intravenous injection of rhEPO.

Under these conditions the test had modest sensitivity to discriminate rhEPO, with only two of nine subjects exceeding an arbitrary cut-off 3.09 SDs beyond the expected population mean. Sensitivity was improved to five out of six subjects if positivity was assessed according to the subject's own previous values rather than a population-based threshold. We conclude that, with further refinement, the dipstick test may supplement existing antidoping tests.
Language eng
DOI 10.1016/j.jpba.2012.04.025
Field of Research 110299 Cardiorespiratory Medicine and Haematology not elsewhere classified
0301 Analytical Chemistry
1115 Pharmacology And Pharmaceutical Sciences
Socio Economic Objective 920101 Blood Disorders
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2012, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075454

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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