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Effect of exercise on the skeletal muscle proteome in patients with type 2 diabetes

Hussey, Sophie E, Sharoff, Carrie G, Garnham, Andrew, Yi, Zhengping, Bowen, Benjamin P, Mandarino, Lawrence J and Hargreaves, Mark 2013, Effect of exercise on the skeletal muscle proteome in patients with type 2 diabetes, Medicine and science in sports and exercise, vol. 45, no. 6, pp. 1069-1076, doi: 10.1249/MSS.0b013e3182814917.

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Title Effect of exercise on the skeletal muscle proteome in patients with type 2 diabetes
Author(s) Hussey, Sophie E
Sharoff, Carrie G
Garnham, Andrew
Yi, Zhengping
Bowen, Benjamin P
Mandarino, Lawrence J
Hargreaves, Mark
Journal name Medicine and science in sports and exercise
Volume number 45
Issue number 6
Start page 1069
End page 1076
Total pages 8
Publisher Lippincott, Williams & Wilkins
Place of publication Philadelphia, Pa.
Publication date 2013-06
ISSN 0195-9131
1530-0315
Keyword(s) Case-Control Studies
Chromatography, High Pressure Liquid
Diabetes Mellitus, Type 2
Exercise
Exercise Test
Female
Humans
Male
Middle Aged
Muscle Proteins
Proteome
Quadriceps Muscle
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Science & Technology
Life Sciences & Biomedicine
Sport Sciences
Summary PURPOSE: Exercise training alters protein abundance in the muscle of healthy individuals, but the effect of exercise on these proteins in patients with type 2 diabetes (T2D) is unknown. The aim of this study was to determine how exercise training alters the skeletal muscle proteome in patients with T2D. METHODS: Biopsies of the vastus lateralis were obtained before and after 4 wk of exercise training in six patients with T2D (54 ± 4 yr; body mass index (BMI), 29 ± 2) and six age- and BMI-matched control subjects (48 ± 2; BMI, 28 ± 3) studied at the baseline. The proteins were identified and quantified using normalized spectral abundance factors by multidimensional high-resolution mass spectrometry. RESULTS: Of the 1329 proteins assigned at the baseline, 438 were present in at least half of all the muscle samples; of these, 15 proteins differed significantly between the patients with T2D and control subjects (P < 0.05). In the diabetic patients, the exercise training altered the abundance of 17 proteins (P < 0.05). Key training adaptations included an increase in proteins of the malate-aspartate shuttle and citric acid cycle, reduced the abundance of glycolytic proteins, and altered the abundance of cytoskeleton proteins. CONCLUSION: The data from this study support the ability of exercise training to alter the abundance of proteins that regulate metabolism and cytoskeletal structure in patients with T2D. These findings open new avenues for future research.
Language eng
DOI 10.1249/MSS.0b013e3182814917
Field of Research 110699 Human Movement and Sports Science not elsewhere classified
1106 Human Movement And Sports Science
Socio Economic Objective 920104 Diabetes
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, the American College of Sports Medicine
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075459

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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