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Cellulases for biomass degradation: comparing recombinant cellulase expression platforms

Garvey, Megan, Klose, Holger, Fischer, Rainer, Lambertz, Camilla and Commandeur, Ulrich 2013, Cellulases for biomass degradation: comparing recombinant cellulase expression platforms, Trends in biotechnology, vol. 31, no. 10, pp. 581-593, doi: 10.1016/j.tibtech.2013.06.006.

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Title Cellulases for biomass degradation: comparing recombinant cellulase expression platforms
Author(s) Garvey, Megan
Klose, Holger
Fischer, Rainer
Lambertz, Camilla
Commandeur, Ulrich
Journal name Trends in biotechnology
Volume number 31
Issue number 10
Start page 581
End page 593
Total pages 13
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2013-10
ISSN 0167-7799
Keyword(s) glycosyl hydrolase
heterologous expression
synthetic biology
consolidated bioprocessing
cellulolytic activity
cellulosome
biomass
industrial processing
Summary Improvement of cellulase expression has the potential to change the nature of the biofuel industry. Increasing the economic feasibility of cellulase systems would significantly broaden the range of practicable biomass conversion, lowering the environmental impact of our civilisations’ fuel needs. Cellulases are derived from certain fungi and bacteria, which are often difficult to culture on an industrial scale. Accordingly, methods to recombinantly express important cellulases and other glycosyl hydrolase (GH) enzymes are under serious investigation. Herein, we examine the latest developments in bacterial, yeast, plant, and fungal expression systems. We discuss current strategies for producing cellulases, and evaluate the benefits and drawbacks in yield, stability, and activity of enzymes from each system, and the overall progress in the field.
Language eng
DOI 10.1016/j.tibtech.2013.06.006
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
06 Biological Sciences
09 Engineering
10 Technology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, Elsevier Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075470

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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