DNA transfer by examination tools--a risk for forensic casework?

Szkuta, Bianca, Harvey, Michelle L., Ballantyne, Kaye N. and van Oorschot, Roland A. H. 2015, DNA transfer by examination tools--a risk for forensic casework?, Forensic science international: genetics, vol. 16, pp. 246-254, doi: 10.1016/j.fsigen.2015.02.004.

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Title DNA transfer by examination tools--a risk for forensic casework?
Author(s) Szkuta, BiancaORCID iD for Szkuta, Bianca orcid.org/0000-0003-2272-5355
Harvey, Michelle L.ORCID iD for Harvey, Michelle L. orcid.org/0000-0002-4047-7845
Ballantyne, Kaye N.
van Oorschot, Roland A. H.
Journal name Forensic science international: genetics
Volume number 16
Start page 246
End page 254
Total pages 9
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-05
ISSN 1878-0326
Keyword(s) Contamination
DNA
Examination tools
Trace
Transfer
Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
Medicine, Legal
Legal Medicine
Summary The introduction of profiling systems with increased sensitivity has led to a concurrent increase in the risk of detecting contaminating DNA in forensic casework. To evaluate the contamination risk of tools used during exhibit examination we have assessed the occurrence and level of DNA transferred between mock casework exhibits, comprised of cotton or glass substrates, and high-risk vectors (scissors, forceps, and gloves). The subsequent impact of such transfer in the profiling of a target sample was also investigated. Dried blood or touch DNA, deposited on the primary substrate, was transferred via the vector to the secondary substrate, which was either DNA-free or contained a target sample (dried blood or touch DNA). Pairwise combinations of both heavy and light contact were applied by each vector in order to simulate various levels of contamination. The transfer of dried blood to DNA-free cotton was observed for all vectors and transfer scenarios, with transfer substantially lower when glass was the substrate. Overall touch DNA transferred less efficiently, with significantly lower transfer rates than blood when transferred to DNA-free cotton; the greatest transfer of touch DNA occurred between cotton and glass substrates. In the presence of a target sample, the detectability of transferred DNA decreased due to the presence of background DNA. Transfer had no impact on the detectability of the target profile, however, in casework scenarios where the suspect profiles are not known, profile interpretation becomes complicated by the addition of contaminating alleles and the probative value of the evidence may be affected. The results of this study reiterate the need for examiners to adhere to stringent laboratory cleaning protocols, particularly in the interest of contamination minimisation, and to reduce the handling of items to prevent intra-item transfer.
Language eng
DOI 10.1016/j.fsigen.2015.02.004
Field of Research 069901 Forensic Biology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30075572

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