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Changes in lipid class content and composition of Isochrysis sp. (T-Iso) grown in batch culture

Nalder, Tim D., Miller, Matthew R. and Packer, Michael A. 2015, Changes in lipid class content and composition of Isochrysis sp. (T-Iso) grown in batch culture, Aquaculture international, vol. 23, no. 5, pp. 1293-1312, doi: 10.1007/s10499-015-9884-9.

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Title Changes in lipid class content and composition of Isochrysis sp. (T-Iso) grown in batch culture
Author(s) Nalder, Tim D.
Miller, Matthew R.
Packer, Michael A.
Journal name Aquaculture international
Volume number 23
Issue number 5
Start page 1293
End page 1312
Total pages 20
Publisher Springer
Place of publication London, Eng.
Publication date 2015-01-30
ISSN 0967-6120
1573-143X
Keyword(s) Aquaculture
DHA
FA biosynthesis
Isochrysis affinis galbana (T-Iso)
Microalgae
Omega-3
Science & Technology
Life Sciences & Biomedicine
Fisheries
FATTY-ACID-COMPOSITION
BIOCHEMICAL-COMPOSITION
LIGHT-INTENSITY
PHAEODACTYLUM-TRICORNUTUM
NANNOCHLOROPSIS-OCULATA
TROPICAL AQUACULTURE
CHEMICAL-COMPOSITION
PAVLOVA-LUTHERI
GALBANA
Summary © 2015, Springer International Publishing Switzerland. For decades, the microalgae Isochrysis spp. have been widely utilised as a live feed in aquaculture practices. This species possesses a number of favourable characteristics, notably its long-chain omega-3 polyunsaturated fatty acid (LC n-3 PUFA) content; primarily docosahexaenoic acid (DHA, 22:6n-3). This article describes the lipid class content and composition of this microalga grown in batch culture, covering the entirety of lag, log and stationary growth phases. The total lipid was highest in the lag phase (27 pg/cell). Total lipid significantly decreased in the exponential growth (7 pg/cell), then steadily increasing for the remainder of growth. The increase in total lipid was due to the accumulation of neutral lipid in the form of triacylglycerides. The DHA content (pg/cell) of the neutral lipid remained relatively unchanged for the duration of growth, with the influx of fatty acids being primarily myristic and palmitic acids. DHA (pg/cell) was found at relatively uniform amounts across all lipid classes. However, the DHA content as a percentage differed greatly between classes. The polar lipid class had a significantly higher DHA content, which peaked at 38 % of all polar lipid in log growth. The primary PUFA species present in the glycolipid class was stearidonic acid (18:4n-3). This work gives an overview of the lipid content and composition of Isochrysis sp. (T-Iso) over the entirety of its growth under batch culture. The lipid profile for this species at different stages of culture provides a basal data set that is useful for comparative studies using this organism.
Language eng
DOI 10.1007/s10499-015-9884-9
Field of Research 070401 Aquaculture
0704 Fisheries Sciences
Socio Economic Objective 830199 Fisheries - Aquaculture not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30076666

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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