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Comparison of cell disruption methods for improving lipid extraction from thraustochytrid strains

Byreddy, Avinesh R., Gupta, Adarsha, Barrow, Colin J. and Puri, Munish 2015, Comparison of cell disruption methods for improving lipid extraction from thraustochytrid strains, Marine drugs, vol. 13, no. 8, pp. 5111-5127, doi: 10.3390/md13085111.

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Title Comparison of cell disruption methods for improving lipid extraction from thraustochytrid strains
Author(s) Byreddy, Avinesh R.
Gupta, Adarsha
Barrow, Colin J.
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Marine drugs
Volume number 13
Issue number 8
Start page 5111
End page 5127
Total pages 17
Publisher MDPI
Place of publication Bern, Switzerland
Publication date 2015-08
ISSN 1660-3397
Keyword(s) DHA
algae
biodiesel
marine
omega-3
solvents
Summary Lipid extraction is an integral part of biodiesel production, as it facilitates the release of fatty acids from algal cells. To utilise thraustochytrids as a potential source for lipid production. We evaluated the extraction efficiency of various solvents and solvent combinations for lipid extraction from Schizochytrium sp. S31 and Thraustochytrium sp. AMCQS5-5. The maximum lipid extraction yield was 22% using a chloroform:methanol ratio of 2:1. We compared various cell disruption methods to improve lipid extraction yields, including grinding with liquid nitrogen, bead vortexing, osmotic shock, water bath, sonication and shake mill. The highest lipid extraction yields were obtained using osmotic shock and 48.7% from Schizochytrium sp. S31 and 29.1% from Thraustochytrium sp. AMCQS5-5. Saturated and monounsaturated fatty acid contents were more than 60% in Schizochytrium sp. S31 which suggests their suitability for biodiesel production.
Language eng
DOI 10.3390/md13085111
Field of Research 060101 Analytical Biochemistry
1115 Pharmacology And Pharmaceutical Sciences
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30076923

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.