Yield optimization of a heterologously expressed novel mouse macrophage protein

Pahuja, Parveen, Srinivasan, Alagiri and Puri, Munish 2013, Yield optimization of a heterologously expressed novel mouse macrophage protein, Biosciences biotechnology research Asia, vol. 10, no. 1, pp. 173-181, doi: 10.13005/bbra/1108.

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Title Yield optimization of a heterologously expressed novel mouse macrophage protein
Author(s) Pahuja, Parveen
Srinivasan, Alagiri
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Journal name Biosciences biotechnology research Asia
Volume number 10
Issue number 1
Start page 173
End page 181
Total pages 9
Publisher Oriental Scientific Publishing
Place of publication Bhopal, India
Publication date 2013-06
ISSN 0973-1245
Summary The large scale whole-genome sequencing projects have resulted in large numbers of un-characterized and un-annotated protein sequences. This presents an opportunity and a challenge to characterise these novel protein sequences. Structural biology has become a widely accepted methodology to help assign functions to such proteins, complementing other cellular and biochemical studies. However, most of these studies require the target protein to be produced in large quantities and in a highly pure and soluble state. The present study is an attempt to maximise production of a recombinant mouse macrophage protein (rMMP) over-expressed heterologously in Escherichia coli. Highest production of biomass and total protein (6.6 mg mL-1) was observed at 37 °C. Maximum cell disruption (89%) was observed during freeze-thawing and subsequent ultrasonication.
Language eng
DOI 10.13005/bbra/1108
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 920411 Nutrition
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, Oriental Scientific Publishing
Persistent URL http://hdl.handle.net/10536/DRO/DU:30076924

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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