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The role of acid-sensing ion channels (ASICs) in epithelial Na+ uptake in adult zebrafish (Danio rerio)

Dymowska, Agnieszka K., Boyle, David, Schultz, Aaron G. and Goss, Greg G. 2015, The role of acid-sensing ion channels (ASICs) in epithelial Na+ uptake in adult zebrafish (Danio rerio), Journal of experimental biology, vol. 218, pp. 1244-1251, doi: 10.1242/​jeb.113118.

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Title The role of acid-sensing ion channels (ASICs) in epithelial Na+ uptake in adult zebrafish (Danio rerio)
Author(s) Dymowska, Agnieszka K.
Boyle, David
Schultz, Aaron G.ORCID iD for Schultz, Aaron G. orcid.org/0000-0002-7333-7646
Goss, Greg G.
Journal name Journal of experimental biology
Volume number 218
Start page 1244
End page 1251
Total pages 8
Publisher Company of Biologists
Place of publication Cambridge, Eng.
Publication date 2015
ISSN 1477-9145
Keyword(s) Gill
Acid-sensing ion channels
Zebrafish
Sodium uptake
Ionoregulation
Summary Acid-sensing ion channels (ASICs) are epithelial Na+ channels gated by external H+. Recently, it has been demonstrated that ASICs play a role in Na+ uptake in freshwater rainbow trout. The current paper investigated the potential involvement of ASICs in Na+ transport in another freshwater fish species, the zebrafish (Danio rerio). Using molecular and histological techniques we found that asic genes and the ASIC4.2 protein are expressed in the gill of adult zebrafish. Immunohistochemistry revealed that mitochondrion-rich cells positive for ASIC4.2 do not co-localize with Na+/K+-ATPase (NKA)-rich cells, but co-localize with cells expressing vacuolar-type H+-ATPase (VHA). Furthermore, pharmacological inhibitors of ASIC and Na+/H+-exchanger (NHEs) significantly reduced uptake of Na+ in adult zebrafish exposed to low Na+ media, but did not cause the same response in individuals exposed to ultra-low Na+ water. Our results suggest that in adult zebrafish ASICs play a role in branchial Na+ uptake in media with low Na+ concentrations and that mechanisms used for Na+ uptake by zebrafish may depend on the Na+ concentration in the acclimation media.
Language eng
DOI 10.1242/​jeb.113118
Field of Research 060602 Animal Physiology - Cell
060604 Comparative Physiology
06 Biological Sciences
11 Medical And Health Sciences
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Company of Biologists
Persistent URL http://hdl.handle.net/10536/DRO/DU:30077137

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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