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Production and immunological analysis of IgE reactive recombinant egg white allergens expressed in Escherichia coli.

Dhanapala, Pathum, Doran, Tim, Tang, Mimi L K and Suphioglu, Cenk 2015, Production and immunological analysis of IgE reactive recombinant egg white allergens expressed in Escherichia coli., Molecular Immunology, vol. 65, no. 1, pp. 104-112, doi: 10.1016/j.molimm.2015.01.006.

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Title Production and immunological analysis of IgE reactive recombinant egg white allergens expressed in Escherichia coli.
Author(s) Dhanapala, Pathum
Doran, Tim
Tang, Mimi L K
Suphioglu, Cenk
Journal name Molecular Immunology
Volume number 65
Issue number 1
Start page 104
End page 112
Total pages 9
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-05
ISSN 1872-9142
Keyword(s) Allergens
Egg allergy
Immunotherapy
Recombinant proteins
Summary IgE-mediated allergy to chicken egg affects a large number of children and adults worldwide. The current management strategy for egg allergy is strict avoidance, however this is impractical due to the presence of eggs in a range of foods and pharmaceutical products including vaccines. Strict avoidance also poses nutritional disadvantages due to high nutritional value of eggs. Allergen specific immunotherapy is being pursued as a curative treatment, in which an allergic individual is gradually exposed to the allergen to induce tolerance. Use of recombinant proteins for immunotherapy has been beneficial due to the purity of the recombinant proteins compared to natural proteins. In this study, we produced IgE reactive recombinant egg white proteins that can be used for future immunotherapy. Using E. coli as an expression system, we successfully produced recombinant versions of Gal d 1, 2 and 3, that were IgE reactive when tested against a pool of egg allergic patients' sera. The IgE reactivity indicates that these recombinant proteins are capable of eliciting an immune response, thus being potential candidates for immunotherapy. We have, for the first time, attempted to produce recombinant versions of all 4 major egg white allergens in E. coli, and successfully produced 3, with only Gal d 4 showing loss of IgE reactivity in the recombinant version. The results suggest that egg allergy in Australian populations may mainly be due to IgE reactivity to Gal d 3 and 4, while Gal d 1 shows higher IgE reactivity. This is the first report of a collective and comparative immunological analysis of all 4 egg white allergens. The significance of this study is the potential use of the IgE reactive recombinant egg white proteins in immunotherapy to treat egg allergic patients.
Language eng
DOI 10.1016/j.molimm.2015.01.006
Field of Research 110701 Allergy
1107 Immunology
Socio Economic Objective 920108 Immune System and Allergy
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30077312

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