The orexinergic system in rainbow trout Oncorhynchus mykiss and its regulation by dietary lipids

Varricchio, Ettorre, Russo, Finizia, Coccia, Elena, Turchini, Giovanni Mario, Francis, David Scott and Paolucci, Marina 2015, The orexinergic system in rainbow trout Oncorhynchus mykiss and its regulation by dietary lipids, Microscopy research and technique, vol. 78, no. 8, pp. 707-714, doi: 10.1002/jemt.22528.

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Title The orexinergic system in rainbow trout Oncorhynchus mykiss and its regulation by dietary lipids
Author(s) Varricchio, Ettorre
Russo, Finizia
Coccia, Elena
Turchini, Giovanni MarioORCID iD for Turchini, Giovanni Mario orcid.org/0000-0003-0694-4283
Francis, David ScottORCID iD for Francis, David Scott orcid.org/0000-0002-4829-6926
Paolucci, Marina
Journal name Microscopy research and technique
Volume number 78
Issue number 8
Start page 707
End page 714
Total pages 8
Publisher Wiley
Place of publication Weinheim, Germany
Publication date 2015-08
ISSN 1097-0029
Keyword(s) gastrointestinal tract
hypothalamus
orexin A
orexin B
orexin receptor
prepro-orexin
rainbow trout
Science & Technology
Life Sciences & Biomedicine
Technology
Anatomy & Morphology
Biology
Microscopy
Life Sciences & Biomedicine - Other Topics
GENE-EXPRESSION
FOOD-INTAKE
TISSUE DISTRIBUTION
MOLECULAR-CLONING
FEEDING-BEHAVIOR
HYPOTHALAMIC NEUROPEPTIDE
PERIPHERAL-TISSUES
LOCOMOTOR-ACTIVITY
DANIO-RERIO
Summary In this study, we report the distribution of orexin A (OXA), orexin B (OXB), and orexin receptor (OX2R) immunoreactive (ir) cells in the hypothalamus and gastrointestinal tract of Oncorhynchus mykiss fed diets with different dietary fatty acid compositions. Trout were fed five iso-energetic experimental diets containing fish oil, or one of four different vegetable oils (olive, sunflower, linseed, and palm oils) as the added dietary lipid source for 12 weeks. OXA, OXB, and OX2R immunoreactive neurons and nervous fibers were identified in the lateral and ventro-medial hypothalamus. OXA, OXB, and OX2R ir cells were found in the mucosa and glands of the stomach and in the mucosa of both the pyloric cecae and intestine. OX2R ir cells were localized in the mucosa layer of both the pyloric cecae and intestine. These immunohistochemical (IHC) results were confirmed via Western blotting. Antibodies against preproorexin (PPO) crossreacted with a band of ∼16 kDa in the hypothalamus, stomach, pyloric cecae, and intestine. Antibodies against OX2R crossreacted with a band of ∼38 kDa in the hypothalamus, pyloric cecae, and intestine. The presence and distribution of OXA, OXB, and OX2R ir cells in the hypothalamus and gastrointestinal tract did not appear to be affected by dietary oils. The presence of orexin system immunoreactive cells in the stomach, pyloric cecae, and intestine of rainbow trout, but not in the enteric nervous system, could suggest a possible role of these peptides as signaling of gastric emptying or endocrine modulation, implying a main local action played by orexins.
Language eng
DOI 10.1002/jemt.22528
Field of Research 070401 Aquaculture
0299 Other Physical Sciences
0601 Biochemistry And Cell Biology
0912 Materials Engineering
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Wiley
Persistent URL http://hdl.handle.net/10536/DRO/DU:30077413

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