Characterization of antibody V segment diversity in the Tasmanian devil (Sarcophilus harrisii)

Ujvari, Beata and Belov, Katherine 2015, Characterization of antibody V segment diversity in the Tasmanian devil (Sarcophilus harrisii), Veterinary immunology and immunopathology, vol. 167, no. 3-4, pp. 156-165, doi: 10.1016/j.vetimm.2015.08.001.

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Title Characterization of antibody V segment diversity in the Tasmanian devil (Sarcophilus harrisii)
Author(s) Ujvari, BeataORCID iD for Ujvari, Beata orcid.org/0000-0003-2391-2988
Belov, Katherine
Journal name Veterinary immunology and immunopathology
Volume number 167
Issue number 3-4
Start page 156
End page 165
Total pages 10
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015-10-15
ISSN 1873-2534
Keyword(s) Heavy and light chains
Immunoglobulin
Marsupial
Tasmanian devil
Variable region
Science & Technology
Life Sciences & Biomedicine
Immunology
Veterinary Sciences
VARIABLE REGION GENES
ANTIGEN-BINDING
IMMUNE-SYSTEM
REPERTOIRE
GENERATION
EVOLUTION
FAMILIES
DISEASE
GENOME
Summary The Tasmanian devil (Sarcophilus harrisii) immune system has recently been under scrutiny because of the emergence of a contagious cancer, which has decimated devil numbers. Here we provide a comprehensive description of the Tasmanian devil immunoglobulin variable regions. We show that heavy chain variable (VH) and light chain variable (VL) repertoires are similar to those described in other marsupial taxa: VL diversity is high, but VH diversity is restricted and belongs only to clan III. As in other mammals, one VH and one Vλ germline family and multiple incomplete Vκ germline sequences were identified in the genome. High Vκ variation was observed in transcripts and we predict that it may have arisen by gene conversion and/or somatic mutations, as it does not appear to have originated from germline variation. Phylogenetic analyses revealed that devil VL gene segments are highly complex and ancient, with some lineages predating the separation of marsupials and eutherians. These results indicate that although the evolutionary history of immune genes lead to the expansions and contractions of immune gene families between different mammalian lineages, some of the ancestral immune gene variants are still maintained in extant species. A high degree of similarity was found between devil and other marsupial VH segments, demonstrating that they originated from a common clade of closely related sequences. The VL families had a higher variation than VH both between and within species. We suggest that, similar to other studied marsupial species, the complex VL segment repertoire compensates for the limited VH diversity in Tasmanian devils.
Language eng
DOI 10.1016/j.vetimm.2015.08.001
Field of Research 069999 Biological Sciences not elsewhere classified
0608 Zoology
0707 Veterinary Sciences
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30077453

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