An acetylcholinesterase inhibition biosensor based on a reduced graphene oxide/silver nanocluster/chitosan nanocomposite for detection of organophosphorus pesticides

Zhang, Yanli, Liu, Hongjun, Yang, Zhongming, Ji, Shunlin, Wang, Junfang, Pang, Pengfei, Feng, Lili, Wang, Hongbin, Wu, Zhan and Yang, Wenrong 2015, An acetylcholinesterase inhibition biosensor based on a reduced graphene oxide/silver nanocluster/chitosan nanocomposite for detection of organophosphorus pesticides, Analytical methods, vol. 7, no. 15, pp. 6213-6219, doi: 10.1039/c5ay01439e.

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Title An acetylcholinesterase inhibition biosensor based on a reduced graphene oxide/silver nanocluster/chitosan nanocomposite for detection of organophosphorus pesticides
Author(s) Zhang, Yanli
Liu, Hongjun
Yang, Zhongming
Ji, Shunlin
Wang, Junfang
Pang, Pengfei
Feng, Lili
Wang, Hongbin
Wu, Zhan
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Journal name Analytical methods
Volume number 7
Issue number 15
Start page 6213
End page 6219
Total pages 7
Publisher Royal Society of Chemistry
Place of publication London, Eng.
Publication date 2015
ISSN 1759-9660
1759-9679
Summary A sensitive electrochemical acetylcholinesterase (AChE) biosensor based on a reduced graphene oxide (rGO) and silver nanocluster (AgNC) modified glassy carbon electrode (GCE) was developed. rGO and AgNC nanomaterials with excellent conductivity, catalytic activity and biocompatibility offered an extremely hydrophilic surface, which facilitated the immobilization of AChE to fabricate the organophosphorus pesticide biosensor. Carboxylic chitosan (CChit) was used as a cross-linker to immobilize AChE on a rGO and AgNC modified GCE. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl. Based on the inhibition effect of organophosphorus pesticides on the AChE activity, using phoxim as a model compound, the inhibition effect of phoxim was proportional to its concentration ranging from 0.2 to 250 nM with a detection limit of 81 pM estimated at a signal-to-noise ratio of 3. The developed biosensor exhibited good sensitivity, stability and reproducibility, thus providing a promising tool for analysis of enzyme inhibitors and direct analysis of practical samples.
Language eng
DOI 10.1039/c5ay01439e
Field of Research 030302 Nanochemistry and Supramolecular Chemistry
0301 Analytical Chemistry
0399 Other Chemical Sciences
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Royal Society of Chemistry
Persistent URL http://hdl.handle.net/10536/DRO/DU:30077792

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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