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Simple and signal-off electrochemical biosensor for mercury(II) based on thymine-mercury-thymine hybridization directly on graphene

Zhang, Yanli, Xie, Jinling, Liu, Yanpei, Pang, Pengfei, Feng, Lili, Wang, Hongbin, Wu, Zhan and Yang, Wenrong 2015, Simple and signal-off electrochemical biosensor for mercury(II) based on thymine-mercury-thymine hybridization directly on graphene, Electrochimica acta, vol. 170, pp. 210-217, doi: 10.1016/j.electacta.2015.04.152.

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Title Simple and signal-off electrochemical biosensor for mercury(II) based on thymine-mercury-thymine hybridization directly on graphene
Author(s) Zhang, Yanli
Xie, Jinling
Liu, Yanpei
Pang, Pengfei
Feng, Lili
Wang, Hongbin
Wu, Zhan
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Journal name Electrochimica acta
Volume number 170
Start page 210
End page 217
Total pages 8
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2015
ISSN 0013-4686
1873-3859
Keyword(s) Mercury (II)
Thymine-mercury-thymine
Graphene
Electrochemical biosensor
Summary Abstract A simple, signal-off and reusable electrochemical biosensor was developed for sensitive and selective detection of mercury(II) based on thymine-mercury(II)-thymine (T-Hg2+-T) complex and the remarkable difference in the affinity of graphene with double strand DNA (ds-DNA) and single strand DNA (ss-DNA). Our system was composed of ferrocene-tagged probe DNA and graphene. Due to the noncovalent assembly, the ferrocene-tagged probe ss-DNA was immobilized on the surface of graphene nanosheets directly and employed to amplify the electrochemical signal. In the presence of Hg2+, the ferrocene-labeled T-rich DNA probe hybridized with target probe to form ds-DNA via the Hg2+-mediated coordination of T-Hg2+-T base pairs. As a result, the duplex DNA complex kept away from the graphene surface due to the weak affinity of graphene and ds-DNA, and the redox current decreased substantially. Meanwhile, the graphene decorated GCE surface was released for the reusability. Under the optimal conditions, the proposed sensor showed a linear concentration range from 25 pM to 10 μM with a detection limit of 5 pM for Hg2+ detection. The strategy afforded exquisite selectivity for Hg2+ against other metal ions in real environmental samples.
Language eng
DOI 10.1016/j.electacta.2015.04.152
Field of Research 030302 Nanochemistry and Supramolecular Chemistry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30077794

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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