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Morphological and molecular investigations of the holocephalan elephant fish nephron: the existence of a countercurrent-like configuration and two separate diluting segments in the distal tubule

Kakumura, Keigo, Takabe, Souichirou, Takagi, Wataru, Hasegawa, Kumi, Konno, Norifumi, Toop, Tes, Bell, Justin D., Donald, John A., Kaneko, Toyoji and Hyodo, Susumu 2015, Morphological and molecular investigations of the holocephalan elephant fish nephron: the existence of a countercurrent-like configuration and two separate diluting segments in the distal tubule, Cell and tissue research, vol. 362, no. 3, pp. 677-688, doi: 10.1007/s00441-015-2234-4.

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Title Morphological and molecular investigations of the holocephalan elephant fish nephron: the existence of a countercurrent-like configuration and two separate diluting segments in the distal tubule
Author(s) Kakumura, Keigo
Takabe, Souichirou
Takagi, Wataru
Hasegawa, Kumi
Konno, Norifumi
Toop, Tes
Bell, Justin D.
Donald, John A.ORCID iD for Donald, John A. orcid.org/0000-0001-5930-2642
Kaneko, Toyoji
Hyodo, Susumu
Journal name Cell and tissue research
Volume number 362
Issue number 3
Start page 677
End page 688
Total pages 12
Publisher Springer
Place of publication New York, N. Y.
Publication date 2015-12
ISSN 1432-0878
Keyword(s) Cartilaginous fish
Diluting segment
Kidney
Membrane transporter
Urea reabsorption
Science & Technology
Life Sciences & Biomedicine
Cell Biology
DOGFISH SQUALUS-ACANTHIAS
COMPUTER-ASSISTED RECONSTRUCTION
ELASMOBRANCH RENAL TUBULE
SKATE RAJA-ERINACEA
SCYLIORHINUS-CANICULUS
CALLORHINCHUS-MILII
FINE-STRUCTURE
MARINE ELASMOBRANCHS
BANDED HOUNDSHARK
TRIAKIS SCYLLIUM
Summary In marine cartilaginous fish, reabsorption of filtered urea by the kidney is essential for retaining a large amount of urea in their body. However, the mechanism for urea reabsorption is poorly understood due to the complexity of the kidney. To address this problem, we focused on elephant fish (Callorhinchus milii) for which a genome database is available, and conducted molecular mapping of membrane transporters along the different segments of the nephron. Basically, the nephron architecture of elephant fish was similar to that described for elasmobranch nephrons, but some unique features were observed. The late distal tubule (LDT), which corresponded to the fourth loop of the nephron, ran straight near the renal corpuscle, while it was convoluted around the tip of the loop. The ascending and descending limbs of the straight portion were closely apposed to each other and were arranged in a countercurrent fashion. The convoluted portion of LDT was tightly packed and enveloped by the larger convolution of the second loop that originated from the same renal corpuscle. In situ hybridization analysis demonstrated that co-localization of Na(+),K(+),2Cl(-) cotransporter 2 and Na(+)/K(+)-ATPase α1 subunit was observed in the early distal tubule and the posterior part of LDT, indicating the existence of two separate diluting segments. The diluting segments most likely facilitate NaCl absorption and thereby water reabsorption to elevate urea concentration in the filtrate, and subsequently contribute to efficient urea reabsorption in the final segment of the nephron, the collecting tubule, where urea transporter-1 was intensely localized.
Language eng
DOI 10.1007/s00441-015-2234-4
Field of Research 1116 Medical Physiology
060603 Animal Physiology - Systems
060807 Animal Structure and Function
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30078003

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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