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Evaluation of the Test-mate ChE (cholinesterase) field kit in acute organophosphorus poisoning

Rajapakse, Bishan N., Thiermann, Horst, Eyer, Peter, Worek, Franz, Bowe, Steven J., Dawson, Andrew H. and Buckley, Nicholas A. 2011, Evaluation of the Test-mate ChE (cholinesterase) field kit in acute organophosphorus poisoning, Annals of emergency medicine, vol. 58, no. 6, pp. 559-564.e6, doi: 10.1016/j.annemergmed.2011.07.014.

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Title Evaluation of the Test-mate ChE (cholinesterase) field kit in acute organophosphorus poisoning
Author(s) Rajapakse, Bishan N.
Thiermann, Horst
Eyer, Peter
Worek, Franz
Bowe, Steven J.ORCID iD for Bowe, Steven J. orcid.org/0000-0003-3813-842X
Dawson, Andrew H.
Buckley, Nicholas A.
Journal name Annals of emergency medicine
Volume number 58
Issue number 6
Start page 559
End page 564.e6
Total pages 12
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2011-12
ISSN 1097-6760
Summary STUDY OBJECTIVE: Measurement of acetylcholinesterase (AChE) is recommended in the management of organophosphorus poisoning, which results in 200,000 deaths worldwide annually. The Test-mate ChE 400 is a portable field kit designed for detecting occupational organophosphorus exposure that measures RBC AChE and plasma cholinesterase (PChE) within 4 minutes. We evaluate Test-mate against a reference laboratory test in patients with acute organophosphorus self-poisoning. METHODS: This was a cross-sectional comparison study of 14 patients with acute organophosphorus poisoning between May 2007 and June 2008. RBC AChE and PChE were measured in 96 and 91 samples, respectively, with the Test-mate ChE field kit and compared with a reference laboratory, using the limits of agreement method (Bland and Altman), κ statistics, and Spearman's correlation coefficients. RESULTS: There was good agreement between the Test-mate ChE and the reference laboratory for RBC AChE. The mean difference (Test-mate-reference) was -0.62 U/g hemoglobin, 95% limits of agreement -10.84 to 9.59 U/g hemoglobin. Good agreement was also observed between the categories of mild, moderate, and severe RBC AChE inhibition (weighted κ 0.85; 95% confidence interval [CI] 0.83 to 0.87). Measurement of PChE also showed good agreement, with a mean difference (Test-mate-reference) of +0.06 U/mL blood, 95% limits of agreement -0.41 to 0.53 U/mL blood. Spearman's correlation coefficients were 0.87 (95% CI 0.81 to 0.91) for RBC AChE and 0.76 (95% CI 0.66 to 0.84) for PChE. Analysis for within-subject correlation of subjects did not change the limits of agreement. CONCLUSION: The Test-mate ChE field kit reliably provides rapid measurement of RBC AChE in acute organophosphorus poisoning.
Language eng
DOI 10.1016/j.annemergmed.2011.07.014
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, American College of Emergency Physicians
Persistent URL http://hdl.handle.net/10536/DRO/DU:30078267

Document type: Journal Article
Collection: Faculty of Health
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