Characterization of two cation diffusion facilitators NpunF0707 and NpunF1794 in Nostoc punctiforme

Hudek, L., Pearson, L., Michalczyk, A. A., Bräu, L., Neilan, B.A. and Ackland, M.L. 2015, Characterization of two cation diffusion facilitators NpunF0707 and NpunF1794 in Nostoc punctiforme, Journal of applied microbiology, vol. 119, no. 5, pp. 1357-1370, doi: 10.1111/jam.12942.

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Title Characterization of two cation diffusion facilitators NpunF0707 and NpunF1794 in Nostoc punctiforme
Author(s) Hudek, L.ORCID iD for Hudek, L.
Pearson, L.
Michalczyk, A. A.ORCID iD for Michalczyk, A. A.
Bräu, L.ORCID iD for Bräu, L.
Neilan, B.A.
Ackland, M.L.ORCID iD for Ackland, M.L.
Journal name Journal of applied microbiology
Volume number 119
Issue number 5
Start page 1357
End page 1370
Total pages 14
Publisher Wiley
Place of publication Weinheim, Germany
Publication date 2015-11
ISSN 1365-2672
Keyword(s) Cyanobacteria
cation diffusion facilitator
metal efflux
zinc homeostasis
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Summary AIMS: To characterize genes involved in maintaining homeostatic levels of zinc in the cyanobacterium Nostoc punctiforme. METHODS AND RESULTS: Metal efflux transporters play a central role in maintaining homeostatic levels of trace elements such as zinc. Sequence analyses of the N. punctiforme genome identified two potential cation diffusion facilitator (CDF) metal efflux transporters, Npun_F0707 (Cdf31) and Npun_F1794 (Cdf33). Deletion of either Cdf31or Cdf33 resulted in increased zinc retention over 3 h. Interestingly, Cdf31(-) and Cdf33(-) mutants showed no change in sensitivity to zinc exposure in comparison with the wild type, suggesting some compensatory capacity for the loss of each other. Using qRT-PCR, a possible interaction was observed between the two cdf's, where the Cdf31(-) mutant had a more profound effect on cdf33 expression than Cdf33(-) did on cdf31. Over-expression of Cdf31 and Cdf33 in ZntA(-) - and ZitB(-) -deficient Escherichia coli revealed function similarities between the ZntA and ZitB of E. coli and the cyanobacterial transporters. CONCLUSIONS: The data presented shed light on the function of two important transporters that regulate zinc homeostasis in N. punctiforme. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows for the first time the functional characterization of two cyanobacterial zinc efflux proteins belonging to the CDF family.
Language eng
DOI 10.1111/jam.12942
Field of Research 100299 Environmental Biotechnology not elsewhere classified
Socio Economic Objective 969999 Environment not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Wiley
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