Characterization of two cation diffusion facilitators NpunF0707 and NpunF1794 in Nostoc punctiforme Hudek, L., Pearson, L., Michalczyk, A. A., Bräu, L., Neilan, B.A. and Ackland, M.L. 2015, Characterization of two cation diffusion facilitators NpunF0707 and NpunF1794 in Nostoc punctiforme, Journal of applied microbiology, vol. 119, no. 5, pp. 1357-1370, doi: 10.1111/jam.12942. Attached Files Name Description MIMEType Size Downloads Title Characterization of two cation diffusion facilitators NpunF0707 and NpunF1794 in Nostoc punctiforme Author(s) Hudek, L. orcid.org/0000-0002-5722-9346 Pearson, L. Michalczyk, A. A. orcid.org/0000-0001-5716-0783 Bräu, L. orcid.org/0000-0001-7105-8339 Neilan, B.A. Ackland, M.L. orcid.org/0000-0002-7474-6556 Journal name Journal of applied microbiology Volume number 119 Issue number 5 Start page 1357 End page 1370 Total pages 14 Publisher Wiley Place of publication Weinheim, Germany Publication date 2015-11 ISSN 1365-2672 Keyword(s) Cyanobacteria cation diffusion facilitator metal efflux zinc zinc homeostasis Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Microbiology MULTIPLE SEQUENCE ALIGNMENT MEMBRANE-PROTEIN TOPOLOGY ZINC TRANSPORTER YIIP ESCHERICHIA-COLI I-TASSER PREDICTION FAMILY CLUSTAL GENE SPECIFICITY Summary AIMS: To characterize genes involved in maintaining homeostatic levels of zinc in the cyanobacterium Nostoc punctiforme. METHODS AND RESULTS: Metal efflux transporters play a central role in maintaining homeostatic levels of trace elements such as zinc. Sequence analyses of the N. punctiforme genome identified two potential cation diffusion facilitator (CDF) metal efflux transporters, Npun_F0707 (Cdf31) and Npun_F1794 (Cdf33). Deletion of either Cdf31or Cdf33 resulted in increased zinc retention over 3 h. Interestingly, Cdf31(-) and Cdf33(-) mutants showed no change in sensitivity to zinc exposure in comparison with the wild type, suggesting some compensatory capacity for the loss of each other. Using qRT-PCR, a possible interaction was observed between the two cdf's, where the Cdf31(-) mutant had a more profound effect on cdf33 expression than Cdf33(-) did on cdf31. Over-expression of Cdf31 and Cdf33 in ZntA(-) - and ZitB(-) -deficient Escherichia coli revealed function similarities between the ZntA and ZitB of E. coli and the cyanobacterial transporters. CONCLUSIONS: The data presented shed light on the function of two important transporters that regulate zinc homeostasis in N. punctiforme. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows for the first time the functional characterization of two cyanobacterial zinc efflux proteins belonging to the CDF family. Language eng DOI 10.1111/jam.12942 Field of Research 100299 Environmental Biotechnology not elsewhere classified Socio Economic Objective 969999 Environment not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2015, Wiley Persistent URL http://hdl.handle.net/10536/DRO/DU:30078505 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Centre for Cellular and Molecular Biology Related Links Link Description Connect to published version Go to link with your DU access privileges   Author URL Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 30 Sep 2015, 15:42:05 EST Thu, 01 Oct 2015, 05:16:27 EST Thu, 01 Oct 2015, 08:33:09 EST Wed, 09 Dec 2015, 13:43:28 EST Thu, 10 Dec 2015, 05:11:12 EST Mon, 21 Dec 2015, 14:06:43 EST Wed, 20 Jan 2016, 10:44:19 EST Filtered Full Citation counts:   Cited 1 times in TR Web of Science Cited 2 times in Scopus Search Google Scholar Access Statistics: 388 Abstract Views, 6 File Downloads  -  Detailed Statistics Created: Wed, 09 Dec 2015, 13:43:28 EST

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