The ZntA-like NpunR4017 plays a key role in maintaining homeostatic levels of zinc in Nostoc punctiforme

Hudek, L., Bräu, L., Michalczyk, A.A., Neilan, B.A., Meeks, J.C. and Ackland, M.L. 2015, The ZntA-like NpunR4017 plays a key role in maintaining homeostatic levels of zinc in Nostoc punctiforme, Applied microbiology and biotechnology, vol. 99, no. 24, pp. 10559-10574, doi: 10.1007/s00253-015-6922-5.

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Title The ZntA-like NpunR4017 plays a key role in maintaining homeostatic levels of zinc in Nostoc punctiforme
Author(s) Hudek, L.ORCID iD for Hudek, L. orcid.org/0000-0002-5722-9346
Bräu, L.ORCID iD for Bräu, L. orcid.org/0000-0001-7105-8339
Michalczyk, A.A.ORCID iD for Michalczyk, A.A. orcid.org/0000-0001-5716-0783
Neilan, B.A.
Meeks, J.C.
Ackland, M.L.ORCID iD for Ackland, M.L. orcid.org/0000-0002-7474-6556
Journal name Applied microbiology and biotechnology
Volume number 99
Issue number 24
Start page 10559
End page 10574
Total pages 16
Publisher Springer
Place of publication New York, N.Y.
Publication date 2015-12
ISSN 1432-0614
Keyword(s) Cyanobacteria
Metal efflux
P-type ATPase
Zinc
Zinc homeostasis
ZntA
Summary Analysis of cellular response to zinc exposure provides insights into how organisms maintain homeostatic levels of zinc that are essential, while avoiding potentially toxic cytosolic levels. Using the cyanobacterium Nostoc punctiforme as a model, qRT-PCR analyses established a profile of the changes in relative mRNA levels of the ZntA-like zinc efflux transporter NpunR4017 in response to extracellular zinc. In cells treated with 18 μM of zinc for 1 h, NpunR4017 mRNA levels increased by up to 1300 % above basal levels. The accumulation and retention of radiolabelled (65)Zn by NpunR4107-deficient and overexpressing strains were compared to wild-type levels. Disruption of NpunR4017 resulted in a significant increase in zinc accumulation up to 24 % greater than the wild type, while cells overexpressing NpunR4107 accumulated 22 % less than the wild type. Accumulation of (65)Zn in ZntA(-) Escherichia coli overexpressing NpunR4017 was reduced by up to 21 %, indicating the capacity for NpunR4017 to compensate for the loss of ZntA. These findings establish the newly identified NpunR4017 as a zinc efflux transporter and a key transporter for maintaining zinc homeostasis in N. punctiforme.
Language eng
DOI 10.1007/s00253-015-6922-5
Field of Research 100299 Environmental Biotechnology not elsewhere classified
Socio Economic Objective 969999 Environment not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2015, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30078506

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