Openly accessible

Characterization of H1N1 swine influenza viruses circulating in Canadian pigs in 2009

Nfon, Charles K., Berhane, Yohannes, Hisanaga, Tamiko, Zhang, Shunzhen, Handel, Katherine, Kehler, Helen, Labrecque, Olivia, Lewis, Nicola S., Vincent, Amy L., Copps, John, Alexandersen, Soren and Pasick, John 2011, Characterization of H1N1 swine influenza viruses circulating in Canadian pigs in 2009, Journal of virology, vol. 85, no. 17, pp. 8667-8679, doi: 10.1128/jvi.00801-11.

Attached Files
Name Description MIMEType Size Downloads

Title Characterization of H1N1 swine influenza viruses circulating in Canadian pigs in 2009
Author(s) Nfon, Charles K.
Berhane, Yohannes
Hisanaga, Tamiko
Zhang, Shunzhen
Handel, Katherine
Kehler, Helen
Labrecque, Olivia
Lewis, Nicola S.
Vincent, Amy L.
Copps, John
Alexandersen, Soren
Pasick, John
Journal name Journal of virology
Volume number 85
Issue number 17
Start page 8667
End page 8679
Total pages 13
Publisher American Society for Microbiology
Place of publication Washington D. C.
Publication date 2011-09
ISSN 0022-538X
1098-5514
Summary The 2009 pandemic H1N1 (pH1N1), of apparent swine origin, may have evolved in pigs unnoticed because of insufficient surveillance. Consequently, the need for surveillance of influenza viruses circulating in pigs has received added attention. In this study we characterized H1N1 viruses isolated from Canadian pigs in 2009. Isolates from May 2009 were comprised of hemagglutinin and neuraminidase (NA) genes of classical SIV origin in combination with the North American triple-reassortant internal gene (TRIG) cassette, here termed contemporary SIV (conSIV) H1N1. These conSIV H1N1 viruses were contiguous with the North American αH1 cluster, which was distinct from the pH1N1 isolates that were antigenically more related to the γH1 cluster. After the initial isolation of pH1N1 from an Alberta pig farm in early May 2009, pH1N1 was found several times in Canadian pigs. These pH1N1 isolates were genetically and antigenically homogeneous. In addition, H1N1 viruses bearing seasonal human H1 and N1 genes together with the TRIG cassette and an NA encoding an oseltamivir-resistance marker were isolated from pigs. The NS gene of one of these seasonal human-like SIV (shSIV) H1N1 isolates was homologous to pH1N1 NS, implicating reassortment between the two strains. Antigenic cross-reactivity was observed between pH1N1 and conSIV but not with shSIV H1N1. In summary, although there was cocirculation of pH1N1 with conSIV and shSIV H1N1 in Canadian pigs after May 2009, there was no evidence supporting the presence of pH1N1 in pigs prior to May 2009. The possibility for further reassortants being generated exists and should be closely monitored.
Language eng
DOI 10.1128/jvi.00801-11
Field of Research 070712 Veterinary Virology
Socio Economic Objective 920120 Zoonoses
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, American Society for Microbiology
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30078685

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 32 times in TR Web of Science
Scopus Citation Count Cited 31 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 443 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Mon, 05 Oct 2015, 12:03:27 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.