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MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle

Wada, Shogo, Kato, Yoshio, Sawada, Shuji, Aizawa, Katsuji, Park, Jong-Hoon, Russell, Aaron P., Ushida, Takashi and Akimoto, Takayuki 2015, MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle, Pflugers Archiv - European journal of physiology, vol. 467, no. 2, pp. 389-398, doi: 10.1007/s00424-014-1517-z.

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Title MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle
Author(s) Wada, Shogo
Kato, Yoshio
Sawada, Shuji
Aizawa, Katsuji
Park, Jong-Hoon
Russell, Aaron P.ORCID iD for Russell, Aaron P. orcid.org/0000-0002-7323-9501
Ushida, Takashi
Akimoto, Takayuki
Journal name Pflugers Archiv - European journal of physiology
Volume number 467
Issue number 2
Start page 389
End page 398
Total pages 10
Publisher Springer
Place of publication Berlin, Germany
Publication date 2015-02
ISSN 0031-6768
1432-2013
Keyword(s) microRNAs (miRNA)
muscle fiber type
peroxisome proliferator-activated receptor gamma
coactivator 1 alpha
endurance performance
Summary Skeletal muscles contain several subtypes of myofibers that differ in contractile and metabolic properties. Transcriptional control of fiber-type specification and adaptation has been intensively investigated over the past several decades. Recently, microRNA (miRNA)-mediated posttranscriptional gene regulation has attracted increasing attention. MiR-23a targets key molecules regulating contractile and metabolic properties of skeletal muscle, such as myosin heavy-chains and peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PGC-1α). In the present study, we analyzed the skeletal muscle phenotype of miR-23a transgenic (miR-23a Tg) mice to explore whether forced expression of miR-23a affects markers of mitochondrial content, muscle fiber composition, and muscle adaptations induced by 4 weeks of voluntary wheel running. When compared with wild-type mice, protein markers of mitochondrial content, including PGC-1α, and cytochrome c oxidase complex IV (COX IV), were significantly decreased in the slow soleus muscle, but not the fast plantaris muscle of miR-23a Tg mice. There was a decrease in type IId/x fibers only in the soleus muscle of the Tg mice. Following 4 weeks of voluntary wheel running, there was no difference in the endurance exercise capacity as well as in several muscle adaptive responses including an increase in muscle mass, capillary density, or the protein content of myosin heavy-chain IIa, PGC-1α, COX IV, and cytochrome c. These results show that miR-23a targets PGC-1α and regulates basal metabolic properties of slow but not fast twitch muscles. Elevated levels of miR-23a did not impact on whole body endurance capacity or exercise-induced muscle adaptations in the fast plantaris muscle.
Language eng
DOI 10.1007/s00424-014-1517-z
Field of Research 111799 Public Health and Health Services not elsewhere classified
0606 Physiology
1116 Medical Physiology
1106 Human Movement And Sports Science
Socio Economic Objective 920499 Public Health (excl. Specific Population Health) not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Springer-Verlag Berlin Heidelberg
Persistent URL http://hdl.handle.net/10536/DRO/DU:30078871

Document type: Journal Article
Collection: Centre for Physical Activity and Nutrition Research
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