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Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats

Maseko, Tebo, Dunshea, Frank Rowland, Howell, Kate, Cho, Hyun-Jung, Rivera, Leni Rose, Furness, John Barton and Ng, Keb 2014, Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats, Nutrients, vol. 6, no. 6, pp. 2478-2492, doi: 10.3390/nu6062478.

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Title Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats
Formatted title Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats
Author(s) Maseko, Tebo
Dunshea, Frank Rowland
Howell, Kate
Cho, Hyun-Jung
Rivera, Leni Rose
Furness, John Barton
Ng, Keb
Journal name Nutrients
Volume number 6
Issue number 6
Start page 2478
End page 2492
Total pages 15
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2014-06-24
ISSN 2072-6643
Keyword(s) Agaricus
Animal Feed
Animals
Gene Expression Regulation, Enzymologic
Glutathione Peroxidase
Hot Temperature
Intestines
Male
Oxidative Stress
Permeability
Random Allocation
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Selenium
Up-Regulation
Se-enriched mushroom
Agaricus bisporus
heat stress
organic Se
gut permeability
glutathione peroxidise-1
glutathione peroxidise-2
α-tocopherol
Summary Dietary effects of organic Se supplementation in the form of Se-enriched Agaricus bisporus mushroom on ileal mucosal permeability and antioxidant selenoenzymes status in heat induced oxidative stress in rats were evaluated. Acute heat stress (40 °C, 21% relative humidity, 90 min exposure) increased ileum baseline short circuit current (Isc; 2.40-fold) and epithelial conductance (Ge; 2.74-fold). Dietary supplementation with Se-enriched A. bisporus (1 µg Se/g feed) reduced (p < 0.05) ileum Isc and Ge during heat stress to 1.74 and 1.91 fold, respectively, indicating protection from heat stress-induced mucosal permeability increase. The expression of ileum glutathione peroxidase (GPx-) 1 and 2 mRNAs were up-regulated (p < 0.05) by 1.90 and 1.87-fold, respectively, for non-heat stress rats on the Se-enriched diet relative to the control. The interplay between heat stress and dietary Se is complex. For rats on the control diet, heat stress alone increased ileum expression of GPx-1 (2.33-fold) and GPx-2 (2.23-fold) relative to thermoneutral conditions. For rats on the Se-enriched diet, heat stress increased (p < 0.05) GPx-1 expression only. Rats on Se-enriched + α-tocopherol diet exhibited increased expression of both genes (p < 0.05). Thus, dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress.
Language eng
DOI 10.3390/nu6062478
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
1111 Nutrition And Dietetics
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30079263

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.