Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells

Cho, Hyun-Jung, Robinson, Eliza S., Rivera, Leni R., McMillan, Paul J., Testro, Adam, Nikfarjam, Mehrdad, Bravo, David M. and Furness, John B. 2014, Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells, Cell and tissue research, vol. 357, no. 1, pp. 63-69, doi: 10.1007/s00441-014-1886-9.

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Title Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells
Author(s) Cho, Hyun-Jung
Robinson, Eliza S.
Rivera, Leni R.
McMillan, Paul J.
Testro, Adam
Nikfarjam, Mehrdad
Bravo, David M.
Furness, John B.
Journal name Cell and tissue research
Volume number 357
Issue number 1
Start page 63
End page 69
Total pages 7
Publisher Springer Verlag
Place of publication Berlin, Germany
Publication date 2014-07
ISSN 0302-766X
Keyword(s) Lcells
gastrointestinal hormones
glucagon like peptide
peptide YY
super-resolution microscopy
Summary A sub-group of enteroendocrine cells (L cells) releasegastrointestinal hormones, GLP-1 and PYY, which havedifferent but overlapping physiological effects, in response tointraluminal nutrients. Whilst their release profiles are notidentical, how the plasma levels of these two hormones aredifferentially regulated is not well understood. We investigatethe possibility that GLP-1 and PYY are in separate storagevesicles. In this study, the subcellular location of GLP-1 andPYY storage organelles is investigated using double-labellingimmunohistochemistry, super resolution microscopy andhigh-resolution confocal microscopy. In all species tested,human, pig, rat and mouse, most cytoplasmic stores thatexhibited GLP-1 or PYY immunofluorescence were distinctfrom each other. The volume occupancy, determined by 3Danalysis, overlapped by only about 10∼20 %. At the lowerresolution achieved by conventional confocal microscopy,there was also evidence of GLP-1 and PYY being in separatestorage compartments but, in subcellular regions where therewere many storage vesicles, separate storage could not beresolved. The results indicate that different storage vesiclesin L cells contain predominantly GLP-1 or predominantlyPYY. Whether GLP-1 and PYY storage vesicles are selectively mobilised and their products are selectively releasedneeds to be determined.
Language eng
DOI 10.1007/s00441-014-1886-9
Field of Research 111699 Medical Physiology not elsewhere classified
1116 Medical Physiology
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Springer-Verlag Berlin Heidelberg
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Document type: Journal Article
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School of Medicine
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